Setup of Quantitative PCR for Oral Neisseria spp. Evaluation in Celiac Disease Diagnosis
| Autor: | Lucia Sacchetti, Paola Salvatore, Giovanna Del Vecchio Blanco, Ilaria Russo, Valeria D'Argenio, Ilaria Granata, Chiara Pagliuca, Carmela Nardelli, Mario Rosario Guarracino, Carolina Ciacci, Maria Valeria Esposito |
|---|---|
| Přispěvatelé: | Esposito, Mv, Nardelli, C, Granata, I, Pagliuca, C, D'Argenio, V, Russo, I, Guarracino, Mr, Salvatore, P, Del Vecchio Blanco, G, Ciacci, C, Sacchetti, L |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_specialty
Clinical Biochemistry Neisseria flavescens Gastroenterology Coeliac disease Pathogenesis Settore MED/12 Internal medicine Neisseria flavescen medicine Celiac disease Diagnostic marker Microbiome neisseria flavescens lcsh:R5-920 biology business.industry medicine.disease biology.organism_classification 16S ribosomal RNA Oral microbiome QPCR Real-time polymerase chain reaction celiac disease oral microbiome diagnostic marker qPCR Neisseria business lcsh:Medicine (General) Dysbiosis |
| Zdroj: | Diagnostics, Vol 10, Iss 1, p 12 (2019) Diagnostics (Basel) 10 (2020). doi:10.3390/diagnostics10010012 info:cnr-pdr/source/autori:Esposito M.V.; Nardelli C.; Granata I.; Pagliuca C.; D'Argenio V.; Russo I.; Guarracino M.R.; Salvatore P.; Del Vecchio Blanco G.; Ciacci C.; Sacchetti L./titolo:Setup of Quantitative PCR for Oral Neisseria spp. Evaluation in Celiac Disease Diagnosis/doi:10.3390%2Fdiagnostics10010012/rivista:Diagnostics (Basel)/anno:2020/pagina_da:/pagina_a:/intervallo_pagine:/volume:10 |
| DOI: | 10.3390/diagnostics10010012 |
| Popis: | Coeliac disease (CD) is a multifactorial autoimmune disorder and gut dysbiosis contributes to its pathogenesis. We previously profiled by 16S rRNA sequencing duodenal and oropharyngeal microbiomes in active CD (a-CD), gluten-free diet (GFD) patients, and controls (CO) and found significantly higher levels of Neisseria spp., with pro-inflammatory activities, in a-CD patients than in the other two groups. In this study, we developed a fast and simple qPCR-based method to evaluate the abundance of the oral Neisseria spp. and the diagnostic performances of the test in CD diagnosis. The Neisseria spp. abundances detected by quantitative PCR (qPCR) were: CO = 0.14, GFD = 0.15, a-CD = 2.08, showing a similar trend to those previously measured by next generation sequencing (NGS). In particular, Neisseria spp. values obtained by both methods were significantly higher (p < 0.001) in a-CD than in the other two groups GFD and CO—the latter almost overlapping. We calculated by ROC curve analysis the threshold of 1.12 ng/μL of Neisseria spp. to discriminate between CO+GFD and a-CD patients with 100% and 96.7% of diagnostic sensitivity and specificity, respectively. In conclusion, our data, if confirmed in other cohorts, suggest the q-PCR evaluation of oral Neisseria spp. could be a fast and simple method to assess CD-associated dysbiosis for diagnostic purposes. |
| Databáze: | OpenAIRE |
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