TCR gene segments from at least one third of Va subfamilies rearrange at the δ locus
| Autor: | Thierry Hercend, Catherine Genevée, Frédéric Triebel, Véronique Chung, Anita Diu |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Delta
Genetics Base Sequence Oligonucleotide Gene Rearrangement delta-Chain T-Cell Antigen Receptor Molecular Sequence Data Immunology T-cell receptor Receptors Antigen T-Cell Locus (genetics) DNA Gene rearrangement Immunogenetics Biology Polymerase Chain Reaction Molecular biology T-Lymphocyte Subsets Humans Amino Acid Sequence Gene Rearrangement alpha-Chain T-Cell Antigen Receptor Molecular Biology Gene Peptide sequence DNA Primers |
| Zdroj: | Molecular Immunology. 31:109-115 |
| ISSN: | 0161-5890 |
| Popis: | Using PCR and an experimentally validated V alpha subfamily-specific oligonucleotide panel (V alpha 1-w29), we have investigated whether the TCR delta chain may increase its combinatorial diversity by using V genes considered as alpha chain-specific. We show that at least 10 distinct human V alpha segments rearrange at the J delta locus, leading to scrambling of the two V gene repertoires. Fifty-five per cent of the V alpha/J delta transcripts characterized here were in frame. The 17 V alpha/C delta chains analysed included an extended CDR3 region with up to 18 aa encoded by the junctional region. In addition, a new J delta segment (J delta 4) has been characterized. Together, these findings demonstrate that combinatorial diversity in the human delta locus is larger than previously thought. |
| Databáze: | OpenAIRE |
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