Human relevance of an in vitro gene signature in HaCaT for skin sensitization
| Autor: | Astrid A. Reus, Henk Van Loveren, Janine Ezendam, Jochem W. van der Veen, Wilfred J. M. Maas, Henny Hodemaekers |
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| Přispěvatelé: | Promovendi ODB, Toxicogenomics, RS: GROW - Oncology, RS: GROW - R1 - Prevention |
| Rok vydání: | 2014 |
| Předmět: |
Adult
Cyclopropanes Keratinocytes Human skin Biology In Vitro Techniques Toxicology Young Adult medicine Gene signature Dinitrochlorobenzene Humans Human relevance Gene Skin integumentary system Local lymph node assay HaCaT Gene Expression Profiling General Medicine Allergens Middle Aged Molecular biology In vitro medicine.anatomical_structure Gene Expression Regulation Cell culture Immunology Dermatitis Allergic Contact Female Keratinocyte Transcriptome Skin sensitization |
| Zdroj: | Toxicology in Vitro, 29(1), 81-84. Elsevier Science |
| ISSN: | 1879-3177 0887-2333 |
| Popis: | The skin sensitizing potential of chemicals is mainly assessed using animal methods, such as the murine local lymph node assay. Recently, an in vitro assay based on a gene expression signature in the HaCaT keratinocyte cell line was proposed as an alternative to these animal methods. Here, the human relevance of this gene signature is assessed through exposure of freshly isolated human skin to the chemical allergens dinitrochlorobenzene (DNCB) and diphenylcyclopropenone (DCP). In human skin, the gene signature shows similar direction of regulation as was previously observed in vitro, suggesting that the molecular processes that drive expression of these genes are similar between the HaCaT cell line and freshly isolated skin, providing evidence for the human relevance of the gene signature. |
| Databáze: | OpenAIRE |
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