Structural basis for ion selectivity and engineering in channelrhodopsins
| Autor: | Michael Rappleye, Andre Berndt |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Neurons
Physics 0303 health sciences Ion selectivity Conducting pathway Cell Membrane fungi Channelrhodopsin Nanotechnology Protein Engineering 03 medical and health sciences 0302 clinical medicine Channelrhodopsins Structural Biology Potassium Animals Humans Neuroscience research Molecular Biology 030217 neurology & neurosurgery 030304 developmental biology |
| Zdroj: | Current Opinion in Structural Biology. 57:176-184 |
| ISSN: | 0959-440X |
| DOI: | 10.1016/j.sbi.2019.04.008 |
| Popis: | Channelrhodopsins have become an integral part of modern neuroscience approaches due to their ability to control neuronal activity in targeted cell populations. The recent determination of several channelrhodopsin X-ray structures now enables us to study their function with unprecedented molecular precision. We will discuss how these insights can guide the engineering of the ion conducting pathway to increase its selectivity for Cl-, Ca2+, and K+ ions and improve the overall conductance. Engineering such channelrhodopsins would further increase their utility in neuroscience research and beyond by controlling a wider range of physiological events. To thoroughly address this issue, we compare channelrhodopsin structures with structural features of voltage and ligand-gated K+, Cl- and Ca2+ channels and discuss how these could be implemented in channelrhodopsins. |
| Databáze: | OpenAIRE |
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