Kinetics of appearance of hemorphins from bovine hemoglobin peptic hydrolysates by a direct coupling of reversed-phase high-performance liquid chromatography and electrospray ionization mass spectrometry
| Autor: | Didier Guillochon, Guy Ricart, Bernadette Coddeville, Naima Nedjar-Arroume, Brigitte Lignot, Renato Froidevaux |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Electrospray
Chromatography Chemistry Electrospray ionization Hydrolysis Organic Chemistry General Medicine Reversed-phase chromatography Mass spectrometry Biochemistry High-performance liquid chromatography Hydrolysate Mass Spectrometry Analytical Chemistry Hemoglobins Kinetics Opioid Peptides Animals Cattle Hemoglobin Amino Acid Sequence Chromatography High Pressure Liquid |
| Zdroj: | Journal of chromatography. A. 873(2) |
| ISSN: | 0021-9673 |
| Popis: | Reversed-phase high-performance liquid chromatography coupled with electrospray ionization mass spectrometry was used to improve the preparation of three opioid peptides (Leu-Val-Val-hemorphin-7, Val-Val-hemorphin-7 and Val-Val-hemorphin-4) resulting from bovine hemoglobin peptic hydrolysates. Optimal conditions for the preparation of these peptides were determined thanks to their kinetic studies of appearance in the course of peptic hydrolyses as a function of degree of hydrolysis of hemoglobin. We propose a low degree of hydrolysis (3%) to prepare Leu-Val-Val-hemorphin-7, a mean degree of hydrolysis (11%) to prepare Val-Val-hemorphin-7 and a high degree of hydrolysis (21%) to prepare Val-Val-hemorphin-4. |
| Databáze: | OpenAIRE |
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