Characterization of the age-dependent intervertebral disc changes in rabbit by correlation between MRI, histology and gene expression

Autor: Claire Vinatier, Olivier Gauthier, Gael Grimandi, Julie Lesoeur, Marion Fusellier, Johann Clouet, Yves Maugars, Martial Masson, Jérôme Guicheux, Marianne Pot-Vaucel, Borhane H. Fellah, Yan Cherel
Přispěvatelé: PresUNAM, Université de Nantes (UN), Service de pharmacie, Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de Rhumatologie, Hôtel-Dieu, Service de chirurgie expérimentale (CRIP-VC), Ecole Nationale Vétérinaire de Nantes-ONIRIS, Service d'imagerie (CRIP-VC), INRA UMR703, Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Nantes-ONIRIS, Graftys SAS, Physiopathologie Animale et bioThérapie du muscle et du système nerveux (PAnTher), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Institut National de la Recherche Agronomique (INRA)-École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), BMC, Ed.
Jazyk: angličtina
Rok vydání: 2011
Předmět:
musculoskeletal diseases
Pathology
medicine.medical_specialty
Aging
lcsh:Diseases of the musculoskeletal system
tissu cellulaire
MRI
HISTOLOGIE
DISQUE VERTEBRAL
MODIFICATION CELLULAIRE
ALTERATION TISSULAIRE
Cell
Down-Regulation
Extracellular matrix
03 medical and health sciences
0302 clinical medicine
Rheumatology
Gene expression
Medicine
Animals
Orthopedics and Sports Medicine
lapin
Intervertebral Disc
Grading (tumors)
030203 arthritis & rheumatology
[SDV.MHEP.RSOA] Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system
Extracellular Matrix Proteins
medicine.diagnostic_test
business.industry
Gene Expression Regulation
Developmental
Magnetic resonance imaging
Histology
Intervertebral disc
biothérapie
Magnetic Resonance Imaging
Up-Regulation
âge
Disease Models
Animal
medicine.anatomical_structure
Animals
Newborn
[SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system
Rabbits
lcsh:RC925-935
business
Nucleus
030217 neurology & neurosurgery
Research Article
Zdroj: BMC Musculoskeletal Disorders
BMC Musculoskeletal Disorders, BioMed Central, 2011, 12 (1), pp.147. ⟨10.1186/1471-2474-12-147⟩
BMC Musculoskeletal Disorders, Vol 12, Iss 1, p 147 (2011)
BMC Musculoskeletal Disorders 147 (12), 1-9. (2011)
ISSN: 1471-2474
Popis: Background The present study was conducted to address whether the intervertebral disc of rabbit could be considered (i) as a valuable model to provide new insights into the tissue and cellular changes of Nucleus pulposus aging and (ii) as an appropriate tool to investigate the efficacy of Nucleus pulposus cell-based biotherapies. Methods Lumbar intervertebral disc from rabbits with increasing ages (1, 6 and 30 month-old) were compared by MRI and histological observation using Pfirrmann's grading and Boos' scoring respectively. The expression of transcripts (COL2A1, AGC1, COL1A1, MMP13, BMP2, MGP and p21) in Nucleus pulposus cells were analysed by quantitative real-time PCR. Results MRI analysis indicated an early age-dependent increase in the Pfirrmann's grading. Histological Boos' scoring was also increased. The analysis of transcript expression levels showed that COL2A1 and AGC1 were down-regulated as a function of age. Conversely, COL1A1, MMP-13, BMP-2, MGP and p21 were significantly up-regulated in the Nucleus pulposus cells of aged rabbit intervertebral disc. Conclusions Our study describes the consistency of the rabbit as a model of intervertebral disc changes as a function of age by correlating tissue alteration with cellular modification measured.
Databáze: OpenAIRE
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