| Autor: |
Chi-Hua Yu, Wei Chen, Yu-Hsuan Chiang, Kai Guo, Zaira Martin Moldes, David L. Kaplan, Markus J. Buehler |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
ACS Biomater Sci Eng |
| ISSN: |
2373-9878 |
| Popis: |
Structural proteins are the basis of many biomaterials and key construction and functional components of all life. Further, it is well-known that the diversity of proteins’ function relies on their local structures derived from their primary amino acid sequences. Here we report a deep learning model to predict the secondary structure content of proteins directly from primary sequences. Understanding the secondary structure content of proteins is crucial to designing proteins with targeted material functions, especially mechanical properties. Using convolutional and recurrent architectures and natural language models, our deep learning model predicts the content of two essential types of secondary structures, alpha helix and beta sheet. The training data is collected from the Protein Data Bank and contains many existing protein geometries. We find that our model can learn the hidden features as patterns of input sequences that can then be directly related to secondary structure content. The alpha helix and beta sheet content predictions show excellent agreement with training data and newly deposited protein structures that were recently identified and which were not included in the original training set. We further demonstrate the features of the model by a search for de novo protein sequences that optimize max/min alpha-helix/beta-sheet content and compare the predictions with folded models of these sequences based on AlphaFold2. Excellent agreement is found, underscoring that our model has predictive potential for designing proteins with specific secondary structures and could be widely applied to biomedical industries, including protein biomaterial designs and regenerative medicine applications. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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