Preferential transfer of mitochondria from endothelial to cancer cells through tunneling nanotubes modulates chemoresistance

Autor: Ludovic Galas, Hamda Al. Thawadi, Arthur Jacob, Massoud Mirshahi, Arash Rafii, Frank Le Foll, Bella S. Guerrouahen, Nadine Abu-Kaoud, Jennifer Pasquier, Pegah Ghiabi, Mahtab Maleki, Shahin Rafii
Přispěvatelé: Weill Medical College of Cornell University [New York], Weill Cornell Medicine [Qatar], Centre de Recherche des Cordeliers (CRC (UMR_S 872)), Université Paris Descartes - Paris 5 (UPD5)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Plate-Forme de Recherche en Imagerie Cellulaire de Haute-Normandie (PRIMACEN), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institute for Research and Innovation in Biomedicine (IRIB), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Sciences Appliquées à L'Environnement (SCALE), Centre National de la Recherche Scientifique (CNRS)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Université Le Havre Normandie (ULH), Normandie Université (NU), Laboratoire d'Ecotoxicologie - Milieux Aquatiques (LEMA), Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU), Stem cell and microenvironment laboratory, Department of Genetic Medicine, Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-High-tech Research Infrastructures for Life Sciences (HeRacLeS), Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Institut National de l'Environnement Industriel et des Risques (INERIS)-Université de Reims Champagne-Ardenne (URCA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), This publication was made possible by grants from the Qatar National Research Fund under its National Priorities Research Program award number NPRP 09-1174-3-291 and NPRP 4-640-1-096., BMC, Ed., Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), LE FOLL, Frank, Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS), Stem Cell and Microenvironment Laboratory, Weill Cornell Medical College in Qatar, Centre de Recherche des Cordeliers ( CRC (UMR_S 872) ), Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Plate-Forme de Recherche en Imagerie Cellulaire de Haute-Normandie ( PRIMACEN ), Université de Rouen Normandie ( UNIROUEN ), Normandie Université ( NU ) -Normandie Université ( NU ) -Institute for Research and Innovation in Biomedicine ( IRIB ), Normandie Université ( NU ) -Normandie Université ( NU ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Université de Rouen Normandie ( UNIROUEN ), Normandie Université ( NU ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Stress Environnementaux et BIOsurveillance des milieux aquatiques ( SEBIO ), Institut National de l'Environnement Industriel et des Risques ( INERIS ) -Université de Reims Champagne-Ardenne ( URCA ) -Université Le Havre Normandie ( ULH ), Normandie Université ( NU ) -Normandie Université ( NU ) -SFR Condorcet, Université de Reims Champagne-Ardenne ( URCA ) -Université de Picardie Jules Verne ( UPJV ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Reims Champagne-Ardenne ( URCA ) -Université de Picardie Jules Verne ( UPJV ) -Centre National de la Recherche Scientifique ( CNRS )
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Cancer cells
Angiogenesis
Endothelial cells
[SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB]
[ SDV.CAN ] Life Sciences [q-bio]/Cancer
0302 clinical medicine
Neoplasms
[SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB]
Medicine(all)
Ovarian Neoplasms
0303 health sciences
Nanotubes
[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology
Neovascularization
Pathologic
General Medicine
Cell biology
Mitochondria
medicine.anatomical_structure
030220 oncology & carcinogenesis
MCF-7 Cells
Female
Tunneling nanotubes
Chemoresistance
Stromal cell
Endothelium
Cell Survival
Intercellular transfer
Green Fluorescent Proteins
Bone Marrow Cells
[SDV.CAN]Life Sciences [q-bio]/Cancer
Biology
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
[SDV.CAN] Life Sciences [q-bio]/Cancer
[ SDV.MHEP ] Life Sciences [q-bio]/Human health and pathology
Cell Line
Tumor
medicine
Cell Adhesion
Humans
Cell adhesion
030304 developmental biology
Biochemistry
Genetics and Molecular Biology(all)
Research
Mesenchymal stem cell
Cell Membrane
Cancer
Mesenchymal Stem Cells
medicine.disease
Coculture Techniques
Cell culture
Drug Resistance
Neoplasm
Cancer cell
[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Zdroj: Journal of Translational Medicine
Journal of Translational Medicine, BioMed Central, 2013, 11, pp.94. ⟨10.1186/1479-5876-11-94⟩
Journal of Translational Medicine, 2013, 11 (1), pp.94. ⟨10.1186/1479-5876-11-94⟩
Journal of Translational Medicine, 2013, 11, pp.94. ⟨10.1186/1479-5876-11-94⟩
Journal of Translational Medicine, BioMed Central, 2013, 11 (1), pp.94. ⟨10.1186/1479-5876-11-94⟩
Journal of Translational Medicine, BioMed Central, 2013, 11 (1), pp.94. 〈10.1186/1479-5876-11-94〉
ISSN: 1479-5876
DOI: 10.1186/1479-5876-11-94⟩
Popis: Our vision of cancer has changed during the past decades. Indeed tumors are now perceived as complex entities where tumoral and stromal components interact closely. Among the different elements of tumor stroma the cellular component play a primordial role. Bone Marrow derived mesenchymal cells (MSCs) are attracted to tumor sites and support tumor growth. Endothelial cells (ECs) play a major role in angiogenesis. While the literature documents many aspects of the cross talk between stromal and cancer cells, the role of direct hetero-cellular contact is not clearly established. Recently, Tunneling nanotubes (TnTs) have been shown to support cell-to-cell transfers of plasma membrane components, cytosolic molecules and organelles within cell lines. Herein, we have investigated the formation of heterocellular TnTs between stromal (MSCs and ECs) and cancer cells. We demonstrate that TnTs occur between different cancer cells, stromal cells and cancer-stromal cell lines. We showed that TnTs-like structure occurred in 3D anchorage independent spheroids and also in tumor explant cultures. In our culture condition, TnTs formation occurred after large membrane adhesion. We showed that intercellular transfers of cytoplasmic content occurred similarly between cancer cells and MSCs or ECs, but we highlighted that the exchange of mitochondria occurred preferentially between endothelial cells and cancer cells. We illustrated that the cancer cells acquiring mitochondria displayed chemoresistance. Our results illustrate the perfusion-independent role of the endothelium by showing a direct endothelial to cancer cell mitochondrial exchange associated to phenotypic modulation. This supports another role of the endothelium in the constitution of the metastatic niche.
Databáze: OpenAIRE
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