Production of cellulose-degrading enzyme on sisal and other agro-industrial residues using a new Brazilian actinobacteria strain Streptomyces sp. SLBA-08
| Autor: | C. L. O. Cerqueira, Aline Simões da Rocha Bispo, D. A. J. Souza, E. P. Macedo, Rosalie Reed Rodrigues Coelho, Rodrigo Pires do Nascimento |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Cellulose-degrading enzyme
biology General Chemical Engineering Sisal bagasse Sugarcane straw lcsh:TP155-156 Sugarcane bagasse Cellulase Straw biology.organism_classification Streptomyces chemistry.chemical_compound chemistry Agronomy Bioenergy biology.protein Streptomyces sp. SLBA-08 Fermentation Food science Cellulose lcsh:Chemical engineering Bagasse computer SISAL computer.programming_language |
| Zdroj: | Brazilian Journal of Chemical Engineering, Vol 30, Iss 4, Pp 729-735 (2013) Brazilian Journal of Chemical Engineering, Volume: 30, Issue: 4, Pages: 729-735, Published: DEC 2013 Brazilian Journal of Chemical Engineering v.30 n.4 2013 Brazilian Journal of Chemical Engineering Associação Brasileira de Engenharia Química (ABEQ) instacron:ABEQ |
| ISSN: | 0104-6632 |
| Popis: | Several types of lignocellulosic raw materials including wheat straw, sisal bagasse and sugarcane bagasse have different contents of cellulosic components. In our studies, aimed at isolating cellulose-degrading actinobacteria from Brazilian soils, a promising strain was selected and identified as Streptomyces sp. SLBA-08. This strain was tested for cellulase production under submerged fermentation in a mineral medium using different carbon sources (sisal bagasse, sugarcane bagasse and straw), as well as ammonium sulphate in different concentrations as nitrogen source. The results showed that medium containing 2.4% (w/v) sisal bagasse and 0.3% (w/v) ammonium sulphate resulted in the highest production of carboxymethylcellulase (1.11 U mL-1), after 48 hours. The pH and temperature profile showed optimal activity at pH 6.0 and 50 °C. As for thermostability, carboxymethylcellulases were tolerant at 50 °C, retaining 70% of the maximal activity even after 2h of incubation. The results obtained indicate that Streptomyces sp. SLBA-08 was capable of producing CMCase using lignocellulosic residues, especially sisal bagasse. |
| Databáze: | OpenAIRE |
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