Stem Cell Expansion and Fate Decision on Liquid Substrates Are Regulated by Self-Assembled Nanosheets
| Autor: | Pavel Novák, Dexu Kong, Lihui Peng, Julien E. Gautrot, Stefania Di Cio |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Keratinocytes Polymers Surface Properties Integrin General Physics and Astronomy Self assembled 03 medical and health sciences Humans General Materials Science Particle Size Cell adhesion Cells Cultured Cell Proliferation chemistry.chemical_classification biology Mesenchymal stem cell General Engineering Substrate (chemistry) Mesenchymal Stem Cells Polymer Nanostructures 030104 developmental biology chemistry Biophysics biology.protein Self-assembly Stem cell Rheology |
| Zdroj: | ACS nano. 12(9) |
| ISSN: | 1936-086X |
| Popis: | The culture of adherent cells is overwhelmingly relying on the use of solid substrates to support cell adhesion. Indeed, it is typically thought that relatively strong bulk mechanical properties (bulk moduli in the range of kPa to GPa) are essential to promote cell adhesion and, in turn, regulate cell expansion and fate decision. In this report, we show that adherent stem cells such as mesenchymal stem cells and primary keratinocytes can be cultured at the surface of liquid substrates and that this phenomenon is mediated by the assembly of polymer nanosheets at the liquid-liquid interface. We use interfacial rheology to quantify this assembly and demonstrate the strong mechanical properties of such nanosheets. Importantly, we show that cell adhesion to such quasi-2D materials is mediated by the classical integrin/acto-myosin machinery, despite the absence of bulk mechanical properties of the underlying liquid substrate. Finally, we show that stem cell proliferation and fate decision are also regulated by the mechanical properties of these self-assembled protein nanosheets. Liquid substrates offer attractive features for the culture of adherent cells and stem cells, and the development of novel stem cell technologies, such as liquid-liquid systems, are particularly well-adapted to automated parallel processing and scale up. |
| Databáze: | OpenAIRE |
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