Isolation and Characterization of Streptococcus mutans Phage as a Possible Treatment Agent for Caries
| Autor: | Reut Kraitman, Shunit Coppenhagen-Glazer, Nurit Beyth, Leron Khalifa, Daniel Gelman, Hadar Ben-Zaken, Ronen Hazan, Gilad Ben-Gal, Sivan Alkalay-Oren |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Saliva phage therapy Streptococcus Phages Phage therapy medicine.medical_treatment 030106 microbiology Genome Viral Dental Caries S. mutans phage medicine.disease_cause Microbiology Article biofilm S. mutans Streptococcus mutans Bacteriophage 03 medical and health sciences bacteriophage Virology medicine Humans biology Pathogenic bacteria biology.organism_classification QR1-502 030104 developmental biology Infectious Diseases Lytic cycle Bacterial virus Bacteria |
| Zdroj: | Viruses Volume 13 Issue 5 Viruses, Vol 13, Iss 825, p 825 (2021) |
| ISSN: | 1999-4915 |
| Popis: | Streptococcus mutans is a key bacterium in dental caries, one of the most prevalent chronic infectious diseases. Conventional treatment fails to specifically target the pathogenic bacteria, while tending to eradicate commensal bacteria. Thus, caries remains one of the most common and challenging diseases. Phage therapy, which involves the use of bacterial viruses as anti-bacterial agents, has been gaining interest worldwide. Nevertheless, to date, only a few phages have been isolated against S. mutans. In this study, we describe the isolation and characterization of a new S. mutans phage, termed SMHBZ8, from hundreds of human saliva samples that were collected, filtered, and screened. The SMHBZ8 genome was sequenced and analyzed, visualized by TEM, and its antibacterial properties were evaluated in various states. In addition, we tested the lytic efficacy of SMHBZ8 against S. mutans in a human cariogenic dentin model. The isolation and characterization of SMHBZ8 may be the first step towards developing a potential phage therapy for dental caries. |
| Databáze: | OpenAIRE |
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