Myostatin is a direct regulator of osteoclast differentiation and its inhibition reduces inflammatory joint destruction in mice
| Autor: | Jessica Bertrand, Thomas Pap, Adelheid Korb-Pap, Peter Paruzel, Daniela Brunert, Svetlana Frank, Kurt Redlich, Denise Beckmann, Berno Dankbar, Corinna Wehmeyer, Silvia Hayer, Athanasios Stratis, Michelle Fennen, Christina Koers-Wunrau |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
Arthritis Osteoclasts Myostatin General Biochemistry Genetics and Molecular Biology Arthritis Rheumatoid Mice Osteoclast Osteogenesis Internal medicine medicine Animals Humans Extracellular Signal-Regulated MAP Kinases biology NFATC Transcription Factors Macrophage Colony-Stimulating Factor RANK Ligand Skeletal muscle Cell Differentiation General Medicine musculoskeletal system medicine.disease medicine.anatomical_structure Endocrinology RANKL GDF11 biology.protein Tumor necrosis factor alpha Transforming growth factor |
| Zdroj: | Nature medicine. 21(9) |
| ISSN: | 1546-170X |
| Popis: | Myostatin is shown to directly promote osteoclast differentiation, and its inhibition improves arthritic bone loss in two mouse models. Myostatin (also known as growth and differentiation factor 8) is a secreted member of the transforming growth factor-β (TGF-β) family that is mainly expressed in skeletal muscle, which is also its primary target tissue. Deletion of the myostatin gene (Mstn) in mice leads to muscle hypertrophy, and animal studies support the concept that myostatin is a negative regulator of muscle growth and regeneration1,2,3,4,5. However, myostatin deficiency also increases bone formation, mainly through loading-associated effects on bone6,7,8,9,10,11. Here we report a previously unknown direct role for myostatin in osteoclastogenesis and in the progressive loss of articular bone in rheumatoid arthritis (RA). We demonstrate that myostatin is highly expressed in the synovial tissues of RA subjects and of human tumor necrosis factor (TNF)-α transgenic (hTNFtg) mice, a model for human RA12. Myostatin strongly accelerates receptor activator of nuclear factor κB ligand (RANKL)-mediated osteoclast formation in vitro through transcription factor SMAD2-dependent regulation of nuclear factor of activated T-cells (NFATC1). Myostatin deficiency or antibody-mediated inhibition leads to an amelioration of arthritis severity in hTNFtg mice, chiefly reflected by less bone destruction. Consistent with these effects in hTNFtg mice, the lack of myostatin leads to increased grip strength and less bone erosion in the K/BxN serum-induced arthritis model in mice. The results strongly suggest that myostatin is a potent therapeutic target for interfering with osteoclast formation and joint destruction in RA. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|