Transplacental transfer of ritodrine and its effect on placental glucose and oxygen consumption in an in vitro human placental cotyledon perfusion
| Autor: | Lea Mor, Jacob Urbach, Sonia Fuchs, Joseph M. Brandes |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Placental cotyledon
medicine.medical_specialty Placenta Drug Evaluation Preclinical Biology In Vitro Techniques Tritium Oxygen Consumption Pregnancy Internal medicine medicine Humans Maternal-Fetal Exchange Fetus Obstetrics and Gynecology Transplacental Metabolism Organ Size Molecular Weight medicine.anatomical_structure Endocrinology Glucose Reproductive Medicine Ritodrine Gestation Female Perfusion Antipyrine medicine.drug |
| Zdroj: | Gynecologic and obstetric investigation. 32(1) |
| ISSN: | 0378-7346 |
| Popis: | Maternofetal transfer of ritodrine and its effect on placental glucose and oxygen consumption were studied using a recycling perfusion of maternal and fetal circulations of an isolated cotyledon of a term human placenta. 3H-labeled ritodrine was introduced into the maternal side of the perfusion system. Transfer was calculated from the linear rise of ritodrine concentrations on the fetal side and was found to be 7.31 +/- 1.02 ng/g placental wet weight/min (SEM) when 1.4 micrograms/ml of ritodrine was used (n = 3) and 14.7 +/- 1.06 ng/g placental wet weight/min when the concentration was 2.8 micrograms/ml (n = 5). The antipyrine transfer rate, 4.1 +/- 0.51 mg/kg/min (n = 8), was used to demonstrate perfusion adequacy and served as an internal standard. Upon computing the mass balance of ritodrine and antipyrine at the end of the experiment, it was found that 17% of ritodrine disappeared from the perfusion system compared to 0.7% of antipyrine (p less than 0.01). The introduction of ritodrine into the perfusion system did not affect placental glucose and oxygen consumption rates of 0.35 +/- 0.01 mumol/g/min (n = 7) and 0.19 +/- 0.013 mumol/g/min (n = 5), respectively. |
| Databáze: | OpenAIRE |
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