Fetal exposure to the maternal microbiota in humans and mice
| Autor: | Catherine H. Plunkett, Debra Brandon, Noelle Younge, Suhail Akhtar, Jessica R. McCann, Amy P. Murtha, Patrick C. Seed, Julie Ballard, Felix Araujo-Perez |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Meconium
0301 basic medicine Placenta Biology Gut flora Andrology Mice 03 medical and health sciences 0302 clinical medicine Pregnancy RNA Ribosomal 16S medicine Animals Humans In Situ Hybridization Fluorescence Mouth Fetus Microbial Viability Bacteria medicine.diagnostic_test Cesarean Section Microbiota Infant Newborn General Medicine biology.organism_classification Gastrointestinal Microbiome Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure In utero 030220 oncology & carcinogenesis Gestation Female Research Article Fluorescence in situ hybridization |
| Zdroj: | JCI Insight. 4 |
| ISSN: | 2379-3708 |
| DOI: | 10.1172/jci.insight.127806 |
| Popis: | Previous studies have demonstrated the presence of microbial DNA in the fetal environment. However, it remains unclear whether this DNA represents viable bacteria and how it relates to the maternal microbiota across body sites. We studied the microbiota of human and mouse dyads to understand these relationships, localize bacteria in the fetus, and demonstrate bacterial viability. In human preterm and full-term mother-infant dyads at the time of cesarean delivery, the oral cavity and meconium of newborn infants born as early as 24 weeks of gestation contained a microbiota that was predicted to originate from in utero sources, including the placenta. Using operative deliveries of pregnant mice under highly controlled, sterile conditions in the laboratory, composition, visualization, and viability of bacteria in the in utero compartment and fetal intestine were demonstrated by 16S rRNA gene sequencing, fluorescence in situ hybridization, and bacterial culture. The composition and predicted source of the fetal gut microbiota shifted between mid- and late gestation. Cultivatable bacteria in the fetal intestine were found during mid-gestation but not late gestation. Our results demonstrate a dynamic, viable mammalian fetal microbiota during in utero development. |
| Databáze: | OpenAIRE |
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