Isolation and Characterization of 13 New Polymorphic Microsatellite Markers in the Phaseolus vulgaris L. (Common Bean) Genome
| Autor: | Zhenhua Hu, Bingcai Wang, Shuzhen Wang, Yi Ding, Chufa Lin, Guolin Zhou, Hongyuan Zhang, Aihua Wang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
DNA
Plant Short Note Population genetics Locus (genetics) Catalysis Inorganic Chemistry Vigna Loss of heterozygosity lcsh:Chemistry Polymorphic Microsatellite Marker Physical and Theoretical Chemistry Amplified Fragment Length Polymorphism Analysis Molecular Biology lcsh:QH301-705.5 Spectroscopy Alleles Genetics Phaseolus Genetic diversity Polymorphism Genetic biology Base Sequence Phaseolus vulgaris L Organic Chemistry food and beverages General Medicine Sequence Analysis DNA genetic diversity microsatellite markers biology.organism_classification Computer Science Applications lcsh:Biology (General) lcsh:QD1-999 Genetic Loci cross-species transferability Amplified fragment length polymorphism Genome Plant Microsatellite Repeats |
| Zdroj: | International Journal of Molecular Sciences, Vol 13, Iss 9, Pp 11188-11193 (2012) International Journal of Molecular Sciences |
| ISSN: | 1422-0067 |
| Popis: | In this study, 13 polymorphic microsatellite markers were isolated from the Phaseolus vulgaris L. (common bean) by using the Fast Isolation by AFLP of Sequence COntaining Repeats (FIASCO) protocol. These markers revealed two to seven alleles, with an average of 3.64 alleles per locus. The polymorphic information content (PIC) values ranged from 0.055 to 0.721 over 13 loci, with a mean value of 0.492, and 7 loci having PIC greater than 0.5. The expected heterozygosity (H(E)) and observed heterozygosity (H(O)) levels ranged from 0.057 to 0.814 and from 0.026 to 0.531, respectively. Cross-species amplification of the 13 prime pairs was performed in its related specie of Vigna unguiculata L. Seven out of all these markers showed cross-species transferability. These markers will be useful for future genetic diversity and population genetics studies for this agricultural specie and its related species. |
| Databáze: | OpenAIRE |
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