The transcriptome and miRNome profiling of glioblastoma tissues and peritumoral regions highlights molecular pathways shared by tumors and surrounding areas and reveals differences between short-term and long-term survivors
| Autor: | Armando Felsani, Anna Moles, Nicola Toschi, Maria Giulia Farace, Silvia Anna Ciafrè, M. Guerrisi, Luigi Grassi, Daniel D'Andrea, Barbara Fazi, Pasquale De Bonis, Daria Sicari, Emilia Luca, Giulio Maira, Carmelo Anile, Annunziato Mangiola |
|---|---|
| Přispěvatelé: | Fazi, Barbara, Felsani, Armando, Grassi, Luigi, Moles, Anna, D'Andrea, Daniel, Toschi, Nicola, Sicari, Daria, De Bonis, Pasquale, Anile, Carmelo, Guerrisi, Maria Giovanna, Luca, Emilia, Farace, Maria Giulia, Maira, Giulio, Ciafré, Silvia Anna, Mangiola, Annunziato |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Adult
Male Cell type Pathology medicine.medical_specialty Settore MED/27 - NEUROCHIRURGIA Brain tumor Biology Deep sequencing NO Transcriptome Extracellular matrix White matter TGFβ microRNA medicine Humans Aged editing glioblastoma peritumoral area Neoplastic Brain Neoplasms Gene Expression Profiling Editing Glioblastoma MicroRNA Peritumoral area TGFß Settore BIO/13 Female Gene Expression Regulation Neoplastic Middle Aged Signal Transduction medicine.disease Gene expression profiling medicine.anatomical_structure Gene Expression Regulation Oncology Research Paper |
| Zdroj: | Scopus-Elsevier Europe PubMed Central Oncotarget |
| Popis: | Glioblastoma multiforme (GBM) is the most common and deadliest primary brain tumor, driving patients to death within 15 months after diagnosis (short term survivors, ST), with the exception of a small fraction of patients (long term survivors, LT) surviving longer than 36 months. Here we present deep sequencing data showing that peritumoral (P) areas differ from healthy white matter, but share with their respective frankly tumoral (C) samples, a number of mRNAs and microRNAs representative of extracellular matrix remodeling, TGFβ and signaling, of the involvement of cell types different from tumor cells but contributing to tumor growth, such as microglia or reactive astrocytes. Moreover, we provide evidence about RNAs differentially expressed in ST vs LT samples, suggesting the contribution of TGF-β signaling in this distinction too. We also show that the edited form of miR-376c-3p is reduced in C vs P samples and in ST tumors compared to LT ones. As a whole, our study provides new insights into the still puzzling distinction between ST and LT tumors, and sheds new light onto that "grey" zone represented by the area surrounding the tumor, which we show to be characterized by the expression of several molecules shared with the proper tumor mass. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|