Expression of neural cell adhesion molecule (N-CAM) in rat islets and its role in islet cell type segregation

Autor: Philippe A. Halban, Vincenzo Cirulli, Lelio Orci, U. Rutishauser, Dominique G. Rouiller, D. Baetens
Rok vydání: 1994
Předmět:
endocrine system
medicine.medical_specialty
Pancreatic Polypeptide-Secreting Cells
Pancreas/ cytology
Cell Adhesion Molecules
Neuronal
Fluorescent Antibody Technique
Enteroendocrine cell
Cell Separation
Biology
Cell Aggregation/physiology
Insulin/analysis
Cadherins/biosynthesis
Immunoglobulin Fab Fragments
Islets of Langerhans
Internal medicine
medicine
Pancreatic polypeptide
Animals
Insulin
Islets of Langerhans/cytology/ metabolism
Pancreas
Cell Aggregation
ddc:616
geography
geography.geographical_feature_category
Pancreatic islets
Cell Biology
Islet
Cadherins
Glucagon
Cell aggregation
Cell biology
Rats
Endocrinology
medicine.anatomical_structure
Glucagon/analysis
Gene Expression Regulation
Neural cell adhesion molecule
Immunoglobulin Fab Fragments/immunology
Cell Adhesion Molecules
Neuronal/ biosynthesis/physiology
Zdroj: Journal of Cell Science, Vol. 107, No 6 (1994) pp. 1429-1436
ISSN: 0021-9533
Popis: Endocrine cell types are non-randomly distributed within pancreatic islets of Langerhans. In the rat, insulin-secreting B-cells occupy the core of the islets and are surrounded by A-, D- and PP-cells, secreting glucagon, somatostatin and pancreatic polypeptide, respectively. Furthermore, dissociated islet cells have the ability in vitro to form aggregates with the same cell-type organization as native islets (pseudoislets). These observations suggest that a differential expression of cell adhesion molecules (CAMs) might characterize B- and non-B-cells (A-, D- and PP-cells), and be in part responsible for the establishment and maintenance of islet architecture. Indirect immunofluorescence using antibodies against CAMs and islet hormones was performed on serial sections of the splenic and duodenal parts of the rat pancreas. Staining for the Ca(2+)-dependent CAM E-cadherin was detected on both exocrine and endocrine tissue and was uniform over the entire islet section, in both pancreatic regions. By contrast, staining for the Ca(2+)-independent neural CAM (N-CAM) was restricted to endocrine tissue and nerve endings. Furthermore, N-CAM staining of endocrine cells was stronger in the islet periphery, a region composed mostly of non-B-cells. Serial sections demonstrate that cells staining strongly for N-CAM in the splenic part correspond to glucagon cells and in the duodenal part to pancreatic polypeptide cells. Within pseudoislets in vitro a stronger staining for N-CAM was also observed on peripheral cells, corresponding to non-B-cells.
Databáze: OpenAIRE
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