Effect of hypertension on angiotensin-(1–7) levels in rats with different angiotensin-I converting enzyme polymorphism
| Autor: | Cristián Palomera, Jorge E. Jalil, Maritza Román, Sergio Lavandero, Jorge Bargetto, María Paz Ocaranza |
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| Rok vydání: | 2006 |
| Předmět: |
Male
medicine.medical_specialty Genotype Peptidyl-Dipeptidase A Left ventricular hypertrophy Rats Mutant Strains General Biochemistry Genetics and Molecular Biology Renovascular hypertension Internal medicine Renin–angiotensin system medicine Animals General Pharmacology Toxicology and Pharmaceutics Polymorphism Genetic Angiotensin 1 biology Chemistry Angiotensin II Radioimmunoassay Angiotensin-converting enzyme General Medicine medicine.disease Angiotensin I converting enzyme Peptide Fragments Rats Endocrinology Rats Inbred Lew Hypertension biology.protein Female Hypertrophy Left Ventricular Angiotensin I |
| Zdroj: | LIFE SCIENCES Artículos CONICYT CONICYT Chile instacron:CONICYT |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/j.lfs.2005.07.026 |
| Popis: | To determine circulating angiotensin-(1-7) [Ang-(1,7)] levels in rats with different angiotensin converting enzyme (ACE) genotypes and to evaluate the effect of hypertension on levels of this heptapeptide, plasma levels of angiotensin II (Ang II) and Ang-(1-7) were determined by HPLC and radioimmunoassay in (a) normotensive F0 and F2 homozygous Brown Norway (BN; with high ACE) or Lewis (with low ACE) rats and (b) in hypertensive F2 homozygous male rats (Goldblatt model). Genotypes were characterized by PCR and plasma ACE activity measured by fluorimetry. Plasma ACE activity was 2-fold higher (p < 0.05) in homozygous BN compared to homozygous Lewis groups. In the Goldblatt groups, a similar degree of hypertension and left ventricular hypertrophy was observed in rats with both genotypes. Plasma Ang II levels were between 300-400% higher (p < 0.05) in the BN than in the Lewis rats, without increment in the hypertensive animals. Plasma Ang-(1-7) levels were 75-87% lower in the BN rats (p < 0.05) and they were significantly higher (p < 0.05) in the hypertensive rats from both genotypes. Plasma levels of Ang II and Ang-(1-7) levels were inversely correlated in the normotensive rats (r = -0.64; p < 0.001), but not in the hypertensive animals. We conclude that there is an inverse relationship between circulating levels of Ang II and Ang-(1-7) in rats determined by the ACE gene polymorphism. This inverse relation is due to genetically determined higher ACE activity. Besides, plasma levels of Ang-(1-7) increase in renovascular hypertension. |
| Databáze: | OpenAIRE |
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