Assessment of established techniques to determine developmental and malignant potential of human pluripotent stem cells
| Autor: | Allison, T.F., Andrews, P.W., Avior, Y., Barbaric, I., Benvenisty, N., Bock, C., Brehm, J., Bruestle, O., Damjanov, I., Elefanty, A., Felkner, D., Gokhale, P.J., Halbritter, F., Healy, L.E., Hu, T.X., Knowles, B.B., Loring, J.F., Ludwig, T.E., Mayberry, R., Micallef, S., Mohamed, J.S., Mueller, F.-J., Mummery, C.L., Nakatsuji, N., Ng, E.S., Oh, S.K.W., O'Shea, O., Pera, M.F., Reubinoff, B., Robson, P., Rossant, J., Schuldt, B.M., Solter, D., Sourris, K., Stacey, G., Stanley, E.G., Suemori, H., Takahashi, K., Yamanaka, S., Initiative, I.S.C. |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
endocrine system diseases Cellular differentiation Cell Culture Techniques Gene Expression General Physics and Astronomy Ectoderm Embryoid body urologic and male genital diseases Regenerative Medicine Mice lcsh:Science Induced pluripotent stem cell Multidisciplinary Stem Cell Research - Induced Pluripotent Stem Cell - Human Endoderm Histological Techniques Teratoma Cell Differentiation female genital diseases and pregnancy complications 3. Good health Cell biology medicine.anatomical_structure Stem cell International Stem Cell Initiative Pluripotent Stem Cells endocrine system Mesoderm Science Biology Article General Biochemistry Genetics and Molecular Biology Cell Line 03 medical and health sciences medicine Animals Humans Stem Cell Research - Embryonic - Human neoplasms Embryoid Bodies Stem Cell Research - Induced Pluripotent Stem Cell Computational Biology General Chemistry Stem Cell Research medicine.disease 030104 developmental biology lcsh:Q Generic health relevance |
| Zdroj: | Nature Communications Nature Communications, 9 Nature communications, vol 9, iss 1 Nature Communications, Vol 9, Iss 1, Pp 1-15 (2018) |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-018-04011-3 |
| Popis: | The International Stem Cell Initiative compared several commonly used approaches to assess human pluripotent stem cells (PSC). PluriTest predicts pluripotency through bioinformatic analysis of the transcriptomes of undifferentiated cells, whereas, embryoid body (EB) formation in vitro and teratoma formation in vivo provide direct tests of differentiation. Here we report that EB assays, analyzed after differentiation under neutral conditions and under conditions promoting differentiation to ectoderm, mesoderm, or endoderm lineages, are sufficient to assess the differentiation potential of PSCs. However, teratoma analysis by histologic examination and by TeratoScore, which estimates differential gene expression in each tumor, not only measures differentiation but also allows insight into a PSC’s malignant potential. Each of the assays can be used to predict pluripotent differentiation potential but, at this stage of assay development, only the teratoma assay provides an assessment of pluripotency and malignant potential, which are both relevant to the pre-clinical safety assessment of PSCs. The International Stem Cell Initiative tests methods in a multisite study to detect pluripotency and teratoma formation (PluriTest, Embryoid Body and Teratoma methods) in human pluripotent stem cells. Here, the authors provide guidelines for their application: only the teratoma assay offers evidence of malignant potential. |
| Databáze: | OpenAIRE |
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