USP16 counteracts mono-ubiquitination of RPS27a and promotes maturation of the 40S ribosomal subunit
| Autor: | Caroline Ashiono, Christian Montellese, Ivo Zemp, Stefanie Jonas, Kerstin Dörner, Paola Picotti, Jasmin van den Heuvel, Andre Melnik, Ludovic C Gillet, Ulrike Kutay |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Ribosomal Proteins
QH301-705.5 Protein subunit Science Ribosome biogenesis ribosome biogenesis translation RPS27A General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Ribosomal protein Humans Eukaryotic Small Ribosomal Subunit Cloning Molecular quality control Biology (General) Ubiquitins 030304 developmental biology Ribosome Subunits Small Eukaryotic 0303 health sciences General Immunology and Microbiology Chemistry General Neuroscience Ubiquitination Translation (biology) General Medicine Cell Biology Ribosomal RNA USP16 3. Good health Cell biology deubiquitinase HEK293 Cells Gene Expression Regulation Protein Biosynthesis Medicine Ubiquitin Thiolesterase 030217 neurology & neurosurgery Biogenesis Gene Deletion Research Article Human |
| Zdroj: | eLife, 9 eLife eLife, Vol 9 (2020) |
| Popis: | Establishment of translational competence represents a decisive cytoplasmic step in the biogenesis of 40S ribosomal subunits. This involves final 18S rRNA processing and release of residual biogenesis factors, including the protein kinase RIOK1. To identify novel proteins promoting the final maturation of human 40S subunits, we characterized pre-ribosomal subunits trapped on RIOK1 by mass spectrometry, and identified the deubiquitinase USP16 among the captured factors. We demonstrate that USP16 constitutes a component of late cytoplasmic pre-40S subunits that promotes the removal of ubiquitin from an internal lysine of ribosomal protein RPS27a/eS31. USP16 deletion leads to late 40S subunit maturation defects, manifesting in incomplete processing of 18S rRNA and retarded recycling of late-acting ribosome biogenesis factors, revealing an unexpected contribution of USP16 to the ultimate step of 40S synthesis. Finally, ubiquitination of RPS27a appears to depend on active translation, pointing at a potential connection between 40S maturation and protein synthesis. |
| Databáze: | OpenAIRE |
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