EQUIFAT: A novel scoring system for the semi-quantitative evaluation of regional adipose tissues in Equidae
Autor: | Charlotte A. Maltin, Patricia A. Harris, C. M. Argo, Dai Grove-White, Philippa K. Morrison |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Physiology lcsh:Medicine Adipose tissue Biochemistry Diagnostic Radiology Fats 0403 veterinary science Medicine and Health Sciences Tissue Distribution Animal Anatomy lcsh:Science Tomography Mammals Multidisciplinary biology Radiology and Imaging 04 agricultural and veterinary sciences Random effects model Lipids Breed Adipose Tissue Physiological Parameters Vertebrates Ponies Anatomy Research Article Rump Imaging Techniques 040301 veterinary sciences Equines Neuroimaging Research and Analysis Methods 03 medical and health sciences Diagnostic Medicine biology.animal Linear regression medicine Animals Pharmacokinetics Horses Obesity Pharmacology business.industry Body Weight lcsh:R Organisms Biology and Life Sciences Reproducibility of Results Equidae medicine.disease Computed Axial Tomography Biological Tissue 030104 developmental biology Amniotes lcsh:Q business Zoology Kappa Neuroscience |
Zdroj: | PLoS ONE, Vol 12, Iss 3, p e0173753 (2017) PLoS ONE PLOS ONE |
ISSN: | 1932-6203 |
Popis: | Anatomically distinct adipose tissues represent variable risks to metabolic health in man and some other mammals. Quantitative-imaging of internal adipose depots is problematic in large animals and associations between regional adiposity and health are poorly understood. This study aimed to develop and test a semi-quantitative system (EQUIFAT) which could be applied to regional adipose tissues. Anatomically-defined, photographic images of adipose depots (omental, mesenteric, epicardial, rump) were collected from 38 animals immediately post-mortem. Images were ranked and depot-specific descriptors were developed (1 = no fat visible; 5 = excessive fat present). Nuchal-crest and ventro-abdominal-retroperitoneal adipose depot depths (cm) were transformed to categorical 5 point scores. The repeatability and reliability of EQUIFAT was independently tested by 24 observers. When half scores were permitted, inter-observer agreement was substantial (average κw: mesenteric, 0.79; omental, 0.79; rump 0.61) or moderate (average κw; epicardial, 0.60). Intra-observer repeatability was tested by 8 observers on 2 occasions. Kappa analysis indicated perfect (omental and mesenteric) and substantial agreement (epicardial and rump) between attempts. A further 207 animals were evaluated ante-mortem (age, height, breed-type, gender, body condition score [BCS]) and again immediately post-mortem (EQUIFAT scores, carcass weight). Multivariable, random effect linear regression models were fitted (breed as random effect; BCS as outcome variable). Only height, carcass weight, omental and retroperitoneal EQUIFAT scores remained as explanatory variables in the final model. The EQUIFAT scores developed here demonstrate clear functional differences between regional adipose depots and future studies could be directed towards describing associations between adiposity and disease risk in surgical and post-mortem situations. |
Databáze: | OpenAIRE |
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