EQUIFAT: A novel scoring system for the semi-quantitative evaluation of regional adipose tissues in Equidae

Autor: Charlotte A. Maltin, Patricia A. Harris, C. M. Argo, Dai Grove-White, Philippa K. Morrison
Rok vydání: 2017
Předmět:
0301 basic medicine
Physiology
lcsh:Medicine
Adipose tissue
Biochemistry
Diagnostic Radiology
Fats
0403 veterinary science
Medicine and Health Sciences
Tissue Distribution
Animal Anatomy
lcsh:Science
Tomography
Mammals
Multidisciplinary
biology
Radiology and Imaging
04 agricultural and veterinary sciences
Random effects model
Lipids
Breed
Adipose Tissue
Physiological Parameters
Vertebrates
Ponies
Anatomy
Research Article
Rump
Imaging Techniques
040301 veterinary sciences
Equines
Neuroimaging
Research and Analysis Methods
03 medical and health sciences
Diagnostic Medicine
biology.animal
Linear regression
medicine
Animals
Pharmacokinetics
Horses
Obesity
Pharmacology
business.industry
Body Weight
lcsh:R
Organisms
Biology and Life Sciences
Reproducibility of Results
Equidae
medicine.disease
Computed Axial Tomography
Biological Tissue
030104 developmental biology
Amniotes
lcsh:Q
business
Zoology
Kappa
Neuroscience
Zdroj: PLoS ONE, Vol 12, Iss 3, p e0173753 (2017)
PLoS ONE
PLOS ONE
ISSN: 1932-6203
Popis: Anatomically distinct adipose tissues represent variable risks to metabolic health in man and some other mammals. Quantitative-imaging of internal adipose depots is problematic in large animals and associations between regional adiposity and health are poorly understood. This study aimed to develop and test a semi-quantitative system (EQUIFAT) which could be applied to regional adipose tissues. Anatomically-defined, photographic images of adipose depots (omental, mesenteric, epicardial, rump) were collected from 38 animals immediately post-mortem. Images were ranked and depot-specific descriptors were developed (1 = no fat visible; 5 = excessive fat present). Nuchal-crest and ventro-abdominal-retroperitoneal adipose depot depths (cm) were transformed to categorical 5 point scores. The repeatability and reliability of EQUIFAT was independently tested by 24 observers. When half scores were permitted, inter-observer agreement was substantial (average κw: mesenteric, 0.79; omental, 0.79; rump 0.61) or moderate (average κw; epicardial, 0.60). Intra-observer repeatability was tested by 8 observers on 2 occasions. Kappa analysis indicated perfect (omental and mesenteric) and substantial agreement (epicardial and rump) between attempts. A further 207 animals were evaluated ante-mortem (age, height, breed-type, gender, body condition score [BCS]) and again immediately post-mortem (EQUIFAT scores, carcass weight). Multivariable, random effect linear regression models were fitted (breed as random effect; BCS as outcome variable). Only height, carcass weight, omental and retroperitoneal EQUIFAT scores remained as explanatory variables in the final model. The EQUIFAT scores developed here demonstrate clear functional differences between regional adipose depots and future studies could be directed towards describing associations between adiposity and disease risk in surgical and post-mortem situations.
Databáze: OpenAIRE
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