Elasticity changes anti-correlate with NO production for human endothelial cells stimulated with TNF-α
| Autor: | Agnieszka Maria Szczygiel, Marta Targosz-Korecka, Marek Szymonski, Grzegorz Brzezinka, Stefan Chlopicki |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
endothelium
Endothelium Physiology Signaling and Cell Physiology medicine.medical_treatment Clinical Biochemistry Cell morphology Microscopy Atomic Force Nitric oxide chemistry.chemical_compound Atomic force microscopy nitric oxide Physiology (medical) medicine Humans Cytoskeleton Actin atomic force microscopy Tumor Necrosis Factor-alpha Endothelial Cells cytoskeleton Actin cytoskeleton Elasticity Cell biology Tumor necrosis factor-α tumor necrosis factor-$\alpha$ medicine.anatomical_structure Cytokine chemistry Tumor necrosis factor alpha Endothelium Vascular |
| Zdroj: | Pflugers Archiv |
| ISSN: | 1432-2013 0031-6768 |
| Popis: | Tumor necrosis factor alpha (TNF-α) is a critical cytokine that is involved in systemic inflammatory response and contributes to the activation of the pro-inflammatory phenotype of the endothelium. In the present study, effects of TNF-α on morphology and elasticity of endothelium in relation to the production of NO and actin fiber reorganization were analyzed in human dermal microvascular endothelial cells. The cells were incubated in MCDB medium solution and stimulated with \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$10\;\text{ng/ml}$\end{document} of TNF-α. Atomic force microscopy measurements have enabled characterization of cell morphology and elastic properties in physiological conditions. The spectrophotometric Griess method was applied to estimate nitric oxide (NO) production of the cells. We demonstrated that TNF-α-induced changes in elasticity of endothelium anti-correlate with NO production and are associated with the reorganization of actin cytoskeleton. |
| Databáze: | OpenAIRE |
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