An analysis of monocytes and dendritic cells differentiated from human peripheral blood monocyte-derived induced pluripotent stem cells
| Autor: | Masaya Hirayama, Masashi Kondo, Takanori Onouchi, Sumito Isogai, Naoki Yamamoto, Kazuyoshi Imaizumi, Noriko Hiramatsu, Shingo Maeda, Takuma Ina |
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| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine medicine.medical_treatment CD14 Induced Pluripotent Stem Cells Primary Cell Culture CX3C Chemokine Receptor 1 Lipopolysaccharide Receptors Biology Monocytes Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine Immune system medicine HLA-DR Humans Induced pluripotent stem cell Molecular Biology Cells Cultured Monocyte Cell Differentiation Dendritic Cells HLA-DR Antigens General Medicine Dendritic cell Immunotherapy Middle Aged Flow Cytometry Healthy Volunteers Transplantation 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Immunology Female Chemokine CCL17 |
| Zdroj: | Medical Molecular Morphology. 53:63-72 |
| ISSN: | 1860-1499 1860-1480 |
| DOI: | 10.1007/s00795-019-00231-8 |
| Popis: | Dendritic cell-based immunotherapy, which uses a patient's own immune cells, can be used for cancer treatment and allergy control, such as autoimmune disease and rejection associated with transplantation. However, these treatments create a burden on patients due to repeated blood collection. We used cell biological analysis of monocytes with few mutations obtained from minimal blood collection for genome recombination. Next, we established human peripheral blood monocyte-derived induced pluripotent stem cells (iPSCs) using a commercial vector and standard culture method. We found that when established iPSCs were induced to differentiate, monocytes showed phagocytic properties and expressed CD14 and CX3CR1. Further, the generated dendritic cells (DCs) expressed CCL17 and highly expressed HLA-DR following the addition of the mite antigen. Taken together, these data show that monocyte-derived iPS cells can be used to differentiate into monocytes and DCs. In addition, the use of these cells can be applied to the pathological analysis of dendritic cell therapy and monocyte diseases. |
| Databáze: | OpenAIRE |
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