New β-galactosidase producers with potential for prebiotic synthesis
| Autor: | Sara C. Silvério, Lígia R. Rodrigues, Eugénia A. Macedo, José A. Teixeira |
|---|---|
| Přispěvatelé: | Universidade do Minho |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Environmental Engineering fructo-oligosaccharides medicine.medical_treatment β-Galactosidase Oligosaccharides Bioengineering Lactose 01 natural sciences Agar plate 03 medical and health sciences chemistry.chemical_compound Lactulose 010608 biotechnology ß-galactosidase medicine galacto-oligosaccharides Food science Waste Management and Disposal 2. Zero hunger chemistry.chemical_classification Science & Technology Renewable Energy Sustainability and the Environment Chromogenic Chemistry Fructosyltransferase activity Prebiotic Galactose General Medicine fungal strains beta-Galactosidase 3. Good health 030104 developmental biology Enzyme Prebiotics Biochemistry Yield (chemistry) lactulose medicine.drug |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| ISSN: | 1873-2976 |
| Popis: | ß-galactosidases (EC 3.2.1.23) are interesting enzymes with potential application in the pharmaceutical and food industry. In this work, a screening study was carried out to identify new fungal sources of -galactosidase. A total of 50 fungi were evaluated using a chromogenic test performed in agar plates. The most promising microorganisms were validated as effective -galactosidase producers under submerged fermentation conditions. The crude -galactosidases were characterized regarding their optimal pH (3.0-5.5) and temperature (45-65 °C). All enzymes showed ability to synthesize lactose-based prebiotics, namely lactulose (maximal yield 3.3%) and a galacto-oligosaccharide (GOS) (maximal yield 20%). Additionally, some enzymatic extracts with fructosyltransferase activity allowed to produce other type of prebiotics, namely fructo-oligosaccharides (FOS). This work, reports for the first time the simultaneous synthesis of different mixtures of GOS (2-15% yield and 0.07-0.5 g/L·h-1 productivity) and FOS (4-30% yield and 0.1-1 g/L·h-1 productivity) by crude extracts exhibiting dual enzymatic activity. This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/ BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER006684),and also by the Project BioTecNorte(NORTE-01-0145-FEDER000004) and the project MultiBiorefinery (POCI-01-0145-FEDER016403) funded by the European Regional Development Fund under thescope ofNorte2020 –Programa OperacionalRegional doNorte.SCS acknowledges her post doc grant (SFRH/BPD/88584/2012) from FCT. EA Macedo is a member of the Associate Laboratory LSRE-LCM, Project POCI-01-0145-FEDER-006984, funded by FEDER through COMPETE2020 – Programa Operacional Competitividade e Internacionalização (POCI) – and by national funds through FCT – Fundação para a Ciência e a Tecnologia. info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
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