Colonization of electrospun polycaprolactone fibers by relevant pathogenic bacterial strains
| Autor: | Carlos Rumbo, Roberto Quesada, Santiago Cuesta-Lopez, Antonio Rinaldi, M. Federica Caso, Juan Antonio Tamayo-Ramos, Lorena Romero-Santacreu |
|---|---|
| Přispěvatelé: | Rinaldi, A. |
| Rok vydání: | 2023 |
| Předmět: |
Acinetobacter baumannii
0301 basic medicine Materials science electrospun polycaprolactone bacterial attachment microfibers nosocomial pathogens foodborne pathogens biofilm foodborne pathogen Polyesters Chemistry Organic 02 engineering and technology medicine.disease_cause Microbiology 03 medical and health sciences chemistry.chemical_compound bacterial attachment biofilm electrospun polycaprolactone foodborne pathogens microfibers nosocomial pathogens Listeria monocytogenes Polylactic acid microfiber medicine General Materials Science Materials Materiales biology Biofilm technology industry and agriculture Química orgánica 021001 nanoscience & nanotechnology biology.organism_classification Antimicrobial nosocomial pathogen Biodegradable polymer 3. Good health 030104 developmental biology chemistry Biofilms Pseudomonas aeruginosa Polycaprolactone 0210 nano-technology Bacteria |
| Zdroj: | Repositorio Institucional de la Universidad de Burgos (RIUBU) instname ACS Applied Materials & Interfaces |
| Popis: | Electrospun biodegradable polymers have emerged as promising materials for their applications in several fields, including biomedicine and food industry. For this reason, the susceptibility of these materials to be colonized by different pathogens is a critical issue for public health, and their study can provide future knowledge to develop new strategies against bacterial infections. In this work, the ability of three pathogenic bacterial species (Pseudomonas aeruginosa, Acinetobacter baumannii, and Listeria monocytogenes) to adhere and form biofilm in electrospun polycaprolactone (PCL) microfibrous meshes was investigated. Bacterial attachment was analyzed in meshes with different microstructure, and comparisons with other materials (borosilicate glass and electrospun polylactic acid (PLA)) fibers were assessed. Analysis included colony forming unit (CFU) counts, scanning electron microscopy (SEM), and crystal violet (CV) staining. All the obtained data suggest that PCL meshes, regardless of their microstructure, are highly susceptible to be colonized by the pathogenic relevant bacteria used in this study, so a pretreatment or a functionalization with compounds that present some antimicrobial activity or antibiofilm properties is highly recommended before their application. Moreover, an experiment designed to simulate a chronic wound environment was used to demonstrate the ability of these meshes to detach biofilms from the substratum where they have developed, thus making them promising candidates to be used in wound cleaning and disinfection. European Union’s H2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 691095 and Junta de Castilla y Leon-FEDER (projects BU079U16 and BU092U16). |
| Databáze: | OpenAIRE |
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