Cyclosporin A in the ocular fluids of uveitis patients following long-term systemic administration
| Autor: | Raffaella Aldigeri, Uta Ceglarek, Francesca Manzotti, Laura Zavota, Paolo Mora, Arturo Carta, Jelka G. Orsoni |
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| Rok vydání: | 2008 |
| Předmět: |
Adult
Male medicine.medical_specialty Lens Capsule Crystalline Administration Oral Cataract Extraction Gastroenterology Group A Group B Autoimmune Diseases Aqueous Humor Uveitis Cellular and Molecular Neuroscience Tandem Mass Spectrometry Internal medicine Cyclosporin a medicine Humans In patient Chromatography High Pressure Liquid Aged Aged 80 and over business.industry Aqueous humour Middle Aged medicine.disease eye diseases Sensory Systems Ophthalmology Tears Immunology Cyclosporine Systemic administration Female sense organs business Immunosuppressive Agents |
| Zdroj: | Graefe's Archive for Clinical and Experimental Ophthalmology. 246:1047-1052 |
| ISSN: | 1435-702X 0721-832X |
| DOI: | 10.1007/s00417-008-0825-4 |
| Popis: | To determine the levels of cyclosporin A (CsA) in tears and the anterior segment of the eye following long-term oral intake for autoimmune diseases. Subjects taking oral CsA to treat relapsing autoimmune ocular inflammation were included in this study. All of the patients had been quiescent for at least 6 months. In patients scheduled for cataract extraction (group A), the CsA levels in the blood, aqueous humour and anterior capsule of the lens were determined. In subjects not requiring surgical intervention (group B), CsA was measured in tears and blood. The samples were analysed using turbulent flow chromatography coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS). There were 19 subjects in group A and 43 subjects in group B. CsA was detectable in all of the tear samples with a mean value of 22.4 ± 20.2 ng/ml and there was a significant positive correlation between the CsA levels in tears and blood (P = 0.012). CsA was not detected in any of the surgical samples. LC-MS/MS proved very sensitive for detecting CsA in low-volume biological samples. CsA was present in human tears in proportion to the blood level after an average of 12 hours from the last oral intake. |
| Databáze: | OpenAIRE |
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