CHEMICAL SENSITIZATION OF CLOSTRIDIUM BOTULINUM SPORES TO RADIATION IN MEAT
| Autor: | D. A. Corlett, K. L. Krabbenhoft, A. W. Anderson, P. R. Elliker |
|---|---|
| Rok vydání: | 1964 |
| Předmět: |
Spores
Meat Sodium chemistry.chemical_element Sodium Chloride medicine.disease_cause General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Mice Sodium nitrate Chlorine medicine Clostridium botulinum Animals Food science General Pharmacology Toxicology and Pharmaceutics Sodium nitrite Spores Bacterial Nitrous acid Nitrates General Immunology and Microbiology Research fungi General Medicine Articles Spore chemistry Food Irradiation Food irradiation Cattle Nuclear chemistry |
| Zdroj: | Applied microbiology. 12 |
| ISSN: | 0003-6919 |
| Popis: | Beef ground round inoculated with 1,000,000 spores of Clostridium botulinum 33-A per gram and containing various additives was exposed to gamma radiation. Spores were inactivated in samples (irradiated at 2.0, 2.5, and 3.0 Mrad) which contained sodium nitrate (1,000 ppm) plus sodium chloride (2.5%). Similar results were obtained when sodium nitrite (200 ppm) was substituted for sodium nitrate, except that there was evidence of spore survival in 1 of 120 cans irradiated at 2.0 Mrad. Spore destruction was based upon the absence of spores and mouse-lethal toxin in meat subcultures made from cans incubated at 35 C for 120 days. Spores were not destroyed when exposed to 2.5 or 3.0 Mrad in the absence of sodium nitrate, sodium nitrite, or sodium chloride. Furthermore, the use of these chemicals individually, together with radiation, was ineffective. The additives alone in the absence of radiation also did not cause spore destruction. Radiation levels of 2.0, 2.5, and 3.0 Mrad, when used with sodium chloride at 1.5 or 2.0% and sodium nitrate at 500 ppm or sodium nitrite at 100 ppm, were ineffective. |
| Databáze: | OpenAIRE |
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