Characterization of a new continuous cell line from the flood water mosquito, Aedes vexans
| Autor: | U. G. Munderloh, T. J. Kurtti, C. A. Mazzacano |
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| Rok vydání: | 1991 |
| Předmět: |
Electrophoresis
Xanthine Dehydrogenase Clinical Biochemistry Biomedical Engineering Bioengineering Biology Moths Isozyme Malate dehydrogenase Cell Line chemistry.chemical_compound Aedes Malate Dehydrogenase Lactate dehydrogenase Animals Aedes vexans chemistry.chemical_classification Genetics L-Lactate Dehydrogenase fungi Esterases Cell Biology biology.organism_classification Molecular biology Isocitrate Dehydrogenase Isoenzymes Isocitrate dehydrogenase Enzyme chemistry Xanthine dehydrogenase Cell culture Karyotyping Larva Biotechnology |
| Zdroj: | Cytotechnology. 5(2) |
| ISSN: | 0920-9069 |
| Popis: | A new cell line, UM-AVE1, was established from embryos of the mosquito Aedes vexans. Banding patterns for the isozymes lactate dehydrogenase (LDH), malate dehydrogenase (MDH), isocitrate dehydrogenase (IDH), xanthine dehydrogenase (XDH), and esterases were compared with those of larval Aedes vexans tissues as well as those of four other mosquito cell lines and one moth cell line. Karyotype analyses confirmed that the dipteran cell lines were not contaminated with lepidopteran cells, because in all mosquito lines the modal number of chromosomes was 6 (= 2n) or 7. Isozyme electrophoresis established a specific profile for each cell line. Two isozymes present in UM-AVE1 (LDH, IDH) were not detected in larvae; this could be a reflection of the different stages used for cell line isolation and enzyme analysis, or lability of sample preparations. It is significant that extracts from UM-AVE1 cells and Aedes vexans larvae had an identical double band for XDH, while all other cell lines examined exhibited only a single band. |
| Databáze: | OpenAIRE |
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