Increased levels of prostaglandins and nitric oxide in esophageal mucosa of children with reflux esophagitis
| Autor: | Alessandra Zicari, Giovanni Corrado, P. Rea, Ettore Cardi, M. Cavaliere, Salvatore Cucchiara, Giuseppe M. Pontieri, Giuseppe Frandina |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1998 |
| Předmět: |
Male
medicine.medical_specialty Biopsy Nitric Oxide Gastroenterology Dinoprostone Nitric oxide chemistry.chemical_compound Esophagus Internal medicine Noxious stimulus Humans Medicine Endoscopy Digestive System Reflux esophagitis Prostaglandin E2 Child Esophagitis Peptic Cells Cultured Mucous Membrane esophagitis gastroesophageal reflux nitric oxide prostaglandins business.industry Esophageal disease Hydrogen-Ion Concentration medicine.disease medicine.anatomical_structure chemistry Pediatrics Perinatology and Child Health Female business Complication Esophagitis medicine.drug |
| Popis: | BACKGROUND Prostaglandin E2 (PGE2) is said to be both protective and detrimental for esophageal mucosal integrity. Nitric oxide (NO) controls several esophageal neuromuscular functions, including relaxation of the lower esophageal sphincter. The purpose of this study was to verify PGE2 and NO levels in esophageal mucosa of children with reflux esophagitis. METHODS The patients were 10 children, age range 7 to 12 years, affected by reflux esophagitis. The control subjects were 10 children, age range 6 to 11 years, with recurrent abdominal pain. Tissue fragments obtained by esophageal biopsies were placed in a culture medium and processed to obtain a cell suspension. Cells were incubated for 24 hours at 37 degrees C. Thereafter, supernatants were collected and divided into aliquots to determine the amounts of PGE2 and NO metabolites. RESULTS Esophageal cells obtained from reflux esophagitis patients synthesize and release a significantly higher (p < 0.01) amount of PGE2 and NO (PGE2 1.9 +/- 0.56 ng/10(6) cells per 24 hours; NO 124.94 +/- 18.36 microM/10(6) cells per 24 hours) than did the control group (PGE2 0.66 +/- 0.14 ng/10(6) cells per 24 hours; NO 68.03 +/- 12.3 microM/10(6) cells per 24 hours). CONCLUSIONS These results suggest that in esophageal mucosa, PGE2 and NO, in low concentrations, are protective, whereas, at high doses, they can be harmful. Higher amounts of PGE2 and NO in the esophageal mucosa of reflux esophagitis patients suggest that similar noxious stimuli trigger the inducible forms of the respective enzyme. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|