Fructo-oligosaccharides and iron bioavailability in anaemic rats: the effects on iron species distribution, ferroportin-1 expression, crypt bifurcation and crypt cell proliferation in the caecum
| Autor: | Célia Colli, Eduardo Henrique Szpak Gaievski, Alexandre Rodrigues Lobo, Eliana Parisi Alvares, Eduardo De Carli |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Male
medicine.medical_specialty Anemia Iron Ferroportin Crypt Oligosaccharides Medicine (miscellaneous) Fructose Plant Roots Caecum Hemoglobins Internal medicine medicine Animals Ferrous Compounds BIODISPONIBILIDADE Intestinal Mucosa Rats Wistar Cation Transport Proteins Cecum Cell Proliferation Nutrition and Dietetics Anemia Iron-Deficiency biology Chemistry Cell growth digestive oral and skin physiology Yacón Biological value biology.organism_classification medicine.disease Gastrointestinal Contents Bioavailability Diphosphates Tracheophyta Prebiotics Endocrinology Biochemistry Food Fortified biology.protein Nutritive Value Brazil Iron Dietary |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1475-2662 0007-1145 |
| DOI: | 10.1017/s0007114514002165 |
| Popis: | The present study investigated the effects of fructo-oligosaccharides (FOS) on the bioavailability of Fe from ferric pyrophosphate (FP), a water-insoluble compound, in Fe-deficient anaemic rats that were subjected to a Hb repletion assay. Male Wistar rats (n64) were fed adequate or low (8 mg/kg) Fe diets for 15 d followed by 1 or 2 weeks of Fe repletion with diets providing 35 mg Fe/kg as ferrous sulphate (FS), FP or FP that was mixed with 7·5 % FOS in the form of yacon flour or Raftilose P95 (RAF), a purified source of FOS. The effects of FOS were observed within the 1st week of the repletion period. Fe bioavailability was improved by FOS supplementation, as measured by Hb regeneration efficiency and hepatic Fe stores, which were more pronounced in the RAF group. Moreover, RAF supplementation resulted in a higher biological value relative to that of the FP group. FOS supplementation resulted in caecal enlargement, in addition to acidification and Fe species redistribution in the caecal contents relative to the control rats. These effects occurred concomitantly with decreased ferroportin (FPN)-1 expression in the caecal mucosa, which was similar in magnitude to that observed in the FS group. Caecum mucosal morphometry was influenced by FOS supplementation, whereas crypt fission and cell proliferation were highest in the caecum of the RAF group. These results reinforce the effects of FOS as Fe bioavailability enhancers in anaemic rats that are sustained by early changes in their caecal environment (decreased mucosal FPN-1 expression and increased Fe absorbability, crypt fission and cellularity). |
| Databáze: | OpenAIRE |
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