Immune response of heifers against a Staphylococcus aureus CP5 whole cell and lysate vaccine formulated with ISCOM Matrix adjuvant
| Autor: | Luis Fernando Calvinho, Cecilia María Camussone, Nazarena Pujato, Carolina Veaute, Bror Morein, Iván Sergio Marcipar |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Staphylococcus aureus
LYSATE CIENCIAS MÉDICAS Y DE LA SALUD medicine.medical_treatment Colony Count Microbial ISCOM MATRIX medicine.disease_cause Statistics Nonparametric Microbiology Biotecnología de la Salud Random Allocation Immune system Adjuvants Immunologic Pregnancy MASTITIS Animals Medicine Mastitis Bovine Opsonin STAPHYLOCOCCUS AUREUS General Veterinary biology Reverse Transcriptase Polymerase Chain Reaction business.industry ISCOM Antibody titer Staphylococcal Infections Antibodies Bacterial IMMUNIZATION Milk Immunization WHOLE CELL Bacterial Vaccines Immunology biology.protein Cytokines RNA Cattle Female Antibody business Adjuvant geographic locations ISCOMs Otras Biotecnologías de la Salud |
| Popis: | Staphylococcus aureus is the most frequently isolated pathogen from bovine intramammary infections worldwide. Commercially available vaccines for mastitis control are composed either of S. aureus lysates or whole-cells formulated with traditional adjuvants. We recently showed the ability of a S. aureus CP5 whole-cell vaccine adjuvanted with ISCOM Matrix to increase specific antibodies production in blood and milk, improving opsonic capacity, compared with the same vaccine formulated with Al(OH)3. However, there is no information about the use of ISCOM Matrix for the formulation of bacterial lysates. The aim of this study was to characterize the innate and humoral immune responses induced by a S. aureus CP5 whole-cell or lysate vaccine, formulated with ISCOM Matrix after immunization of pregnant heifers. Both immunogens stimulated strong humoral immune responses in blood and milk, raising antibodies that increased opsonic capacity. Lysate formulation generated a higher and longer lasting antibody titer and stimulated a higher expression of regulatory and pro-inflammatory cytokines compared with the whole-cell vaccine. Fil: Camussone, Cecilia María. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina Fil: Veaute, Carolina Melania Isabel. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina Fil: Pujato, Nazarena. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina Fil: Morein, Bror. Uppsala University; Suecia Fil: Marcipar, Iván Sergio. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina Fil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina |
| Databáze: | OpenAIRE |
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