Anti-infection tissue engineering construct treating osteomyelitis in rabbit tibia
| Autor: | Jianzhong Xu, Qianbo Chen, Fei Luo, Bianba Luobu, Junchao Xing, Tianyong Hou, Zhiqiang Li, Huiyong Jin |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
medicine.medical_specialty
medicine.medical_treatment Biomedical Engineering Bioengineering Bone healing Bone grafting Biochemistry Fibrin Biomaterials Tissue engineering Anti-Infective Agents Vancomycin Medicine Animals Tibia Fracture Healing biology Tissue Scaffolds business.industry Osteomyelitis Mesenchymal stem cell medicine.disease Combined Modality Therapy Surgery Transplantation Tibial Fractures Treatment Outcome Delayed-Action Preparations Bone Substitutes biology.protein Rabbits business |
| Zdroj: | Tissue engineering. Part A. 19(1-2) |
| ISSN: | 1937-335X |
| Popis: | Bone defect complicated by infection remains a major challenge in orthopedic surguries, and bone grafting for primary repair often associates with high failure rate. The rapid progress in the research spectrums of tissue-engineered bone and antibiotic delivery systems bring hope to solve this issue. Herein, we evaluated the local anti-infective and osteogenic potential of an injectable anti-infection tissue-engineered construct, which includes a fibrin gel scaffold and vancomycin alginate beads (Vanco-AB) to form composites, in the treatment of chronic osteomyelitis with bone defect in rabbit tibia. The infected bone defect model of rabbit tibia was established. Then, the bone defects in the proximal tibial metaphysis were implanted with the constructed composites, containing different combinations of mesenchymal stem cells and Vanco-AB. The in vivo capacities of anti-infection and local osteogenesis of the grafts were determined using radiographic assessment, histopathological observation, and microorganism cultures. Results showed that the injectable anti-infection tissue-engineered construct, comprising a fibrin gel scaffold and Vanco-AB led to efficient eradication of bacteria. At 1 and 3 months after transplantation, the radiographic assessment and microbiological examination demonstrated that the sustained antibiotic release by Vanco-AB significantly decreased the Norden scores of osteomyelitis, generated negative results for the presence of bacteria, and reduced the relapse of osteomyelitis. Meanwhile, tissue-engineered construct implanted in one-stage promoted local bone repair and reconstruction, and it exhibited more apparent osteogenic potential, compared to the control group (without Vanco-AB). In conclusion, the current study achieved the primary repair of bone defect with infection, thus providing an alternative treatment strategy for infected bone defect, which occurs commonly in chronic osteomyelitis. |
| Databáze: | OpenAIRE |
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