Direct detection of genomic DNA with fluidic force discrimination assays
| Autor: | Shawn P. Mulvaney, Cy R. Tamanaha, Lloyd J. Whitman, C.N. Ibe |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
DNA
Bacterial biology Strain (chemistry) Sonication Bacillus thuringiensis Biophysics Cell Biology Microfluidic Analytical Techniques biology.organism_classification Biochemistry Molecular biology Bacillus anthracis chemistry.chemical_compound genomic DNA Restriction enzyme chemistry Fluidics Molecular Biology Biosensor Genome Bacterial DNA |
| Zdroj: | Analytical Biochemistry. 392:139-144 |
| ISSN: | 0003-2697 |
| Popis: | Herein, we describe the direct detection of genomic DNA using fluidic force discrimination (FFD) assays. Starting with extracted bacterial DNA, samples are fragmented by restriction enzymes or sonication, then thermocycled in the presence of blocking and labeling sequences, and finally detected with microbead-based FFD assays. Both strain and species identification of extracted Bacillus DNA have been demonstrated in |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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