Distinct Contribution of Adult-Born Hippocampal Granule Cells to Context Encoding
| Autor: | Alexander R. Goldberg, Joseph Tsai, Matthew Lovett-Barron, Patrick Kaifosh, Jeffrey D. Zaremba, Mazen A. Kheirbek, Liam Drew, Nathan Danielson, Attila Losonczy, René Hen, Christine A. Denny, Elizabeth M. Balough |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Neurogenesis Cellular differentiation Optogenetics Biology Hippocampal formation Hippocampus Mice 03 medical and health sciences 0302 clinical medicine Calcium imaging Animals Gene silencing Neurons Communication business.industry General Neuroscience Dentate gyrus Granule (cell biology) Cell Differentiation Microscopy Fluorescence Multiphoton 030104 developmental biology Dentate Gyrus Calcium business Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Neuron. 90:101-112 |
| ISSN: | 0896-6273 |
| Popis: | Adult-born granule cells (abGCs) have been implicated in cognition and mood; however, it remains unknown how these cells behave in vivo. Here, we have used two-photon calcium imaging to monitor the activity of young abGCs in awake behaving mice. We find that young adult-born neurons fire at a higher rate in vivo but paradoxically exhibit less spatial tuning than their mature counterparts. When presented with different contexts, mature granule cells underwent robust remapping of their spatial representations, and the few spatially tuned adult-born cells remapped to a similar degree. We next used optogenetic silencing to confirm the direct involvement of abGCs in context encoding and discrimination, consistent with their proposed role in pattern separation. These results provide the first in vivo characterization of abGCs and reveal their participation in the encoding of novel information. |
| Databáze: | OpenAIRE |
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