No detectable beneficial systemic immunomodulatory effects of a specific synbiotic mixture in infants with atopic dermatitis

Autor: L B, van der Aa, R, Lutter, H S A, Heymans, B S, Smids, T, Dekker, W M C, van Aalderen, J H, Sillevis Smitt, L M J, Knippels, J, Garssen, A J, Nauta, A B, Sprikkelman, M A, van Houten
Přispěvatelé: AII - Amsterdam institute for Infection and Immunity, General Paediatrics, Pulmonology, Experimental Immunology, Other departments, Paediatric Pulmonology, Other Research, Dermatology, AGEM - Amsterdam Gastroenterology Endocrinology Metabolism, APH - Amsterdam Public Health, CCA -Cancer Center Amsterdam, Paediatric Oncology
Jazyk: angličtina
Rok vydání: 2012
Předmět:
Male
Synbiotics
medicine.medical_treatment
ved/biology.organism_classification_rank.species
chemokines
Immunoglobulin E
T-Lymphocytes
Regulatory
FOOD ALLERGY
regulatory T cells
MEMORY T-CELLS
Immunology and Allergy
Bifidobacterium breve
atopic dermatitis
biology
infants
IMMUNE-RESPONSES
MURINE MODEL
Atopic dermatitis
Infant Formula
CONTROLLED TRIAL
medicine.anatomical_structure
Cytokine
PROBIOTIC BACTERIA
Female
Regulatory T cell
Immunology
LACTIC-ACID BACTERIA
Dermatitis
Atopic
Allergic inflammation
Immunomodulation
Double-Blind Method
medicine
Humans
Immunologic Factors
synbiotics
MONONUCLEAR-CELLS
LACTOBACILLUS-GG
ved/biology
business.industry
Chemokine CCL27
Infant
Newborn
Infant
IN-VITRO
medicine.disease
cytokines
probiotics
Immunoglobulin G
biology.protein
Bifidobacterium
Chemokine CCL17
Interleukin-5
prebiotics
business
Ex vivo
Zdroj: Clinical and experimental allergy, 42(4), 531-539. Wiley-Blackwell
Clinical and Experimental Allergy, 42(4), 531-539. Wiley
ISSN: 0954-7894
Popis: Background In a murine model of allergic inflammation, Bifidobacterium breve M-16V has been shown to reduce IL-4 and IgE by inducing IL-10 and IFN-gamma. However, it remains unknown whether this strain has the same effect in humans with allergic disease. Objective To determine the effects of Bifidobacterium breve M-16V combined with a prebiotic oligosaccharide mixture (synbiotic) on atopic markers, ex vivo cytokine production by peripheral blood mononuclear cells (PBMCs) and circulating regulatory T cell percentage in infants with atopic dermatitis.Methods In a double-blind, placebo-controlled multi-centre trial, 90 infants with atopic dermatitis, age Results There were no significant differences between the synbiotic and the placebo group in IL-5, IgG1, IgG4, CTACK and TARC levels and ex vivo cytokine production by antiCD3/anti-CD28-stimulated PBMCs. With allergen-specific stimuli, we found a decreased IL-12p40/70 and IL-12p70 production in response to egg allergen (P = 0.04 and P = 0.01, respectively) and decreased IL-12p70 production in response to peanut allergen (P = 0.003) in the synbiotic compared with the placebo group. Circulating regulatory T cell percentage did not significantly differ between the groups.Conclusions and Clinical Relevance This synbiotic mixture has no detectable effect on plasma levels of the analysed atopic disease markers, ex vivo cytokine production and circulating regulatory T cell percentage in infants with atopic dermatitis, besides down-regulation of IL-12 production in egg-and peanut-stimulated PBMCs. These results do not support the use of this synbiotic in clinical practice.
Databáze: OpenAIRE
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