Contribution of efflux pumps, porins, and β-lactamases to multidrug resistance in clinical isolates of Acinetobacter baumannii
| Autor: | Jerónimo Pachón, María Tomás, Germán Bou, Carlos Rumbo, Luis Martínez-Martínez, María M. López, Jesús Rodríguez-Baño, José Miguel Cisneros, C. Ruiz de Alegría, Almudena Jiménez Pascual, Felipe Fernández-Cuenca, Eva Gato, Jordi Vila |
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| Přispěvatelé: | Antimicrobials (GEIH-GEMARA) from the Spanish Society of Clinical Microbiology and Infectious Diseases (SEIMC), The Spanish Network for Research in Infectious Diseases (REIPI), [Rumbo,C, Gato,E, López,M, Bou,G, Tomás,M] Department of Microbiology, Complejo Hospitalario Universitario a Coruña-INIBIC, La Coruña, Spain.[Ruiz de Alegría,C, Martínez-Martínez,L] Department of Clinical Microbiology, Hospital Universitario Marqués de Valdecilla-IFIMAV, Santander, Spain.[Fernández-Cuenca,F, Rodríguez-Baño,J, Pascual,A] Clinical Unit of Infectious Diseases and Microbiology, Hospital Universitario Virgen Macarena, Seville, Spain.[Vila,J] Department of Clinical Microbiology, Hospital Clinic-CRESIB, School of Medicine, University of Barcelona, Barcelona, Spain.[Cisneros,JM] Institute of Biomedicine of Seville (IBiS), University Hospital Virgen del Rocío/CSIC/University of Seville, Seville, Spain., Research in our laboratories is financially supported by grants from the Instituto de Salud Carlos III, the Spanish Network for Research in Infectious Diseases (REIPI RD 06/0008/0025), the Fondo de Investigaciones Sanitarias (PI08/1368 and PS09/00687 to G.B. and PI10/00056 to M.T.), SERGAS (PS07/90), and the Xunta de Galicia (07CSA050916PR). C.R. is funded by a doctoral grant (PFIS) from the Instituto de Salud Carlos III, and M.T. is supported by the Instituto de Salud Carlos III (program Miguel Servet), both from the Ministerio de Economia y Competitividad., Universidad de Sevilla. Departamento de Medicina |
| Rok vydání: | 2013 |
| Předmět: |
Acinetobacter baumannii
Aminoglicósidos Porinas Tigecycline Drug resistance Quinolones Carbapenémicos Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings] Chemicals and Drugs::Organic Chemicals::Hydrocarbons::Hydrocarbons Cyclic::Hydrocarbons Aromatic::Polycyclic Hydrocarbons Aromatic::Naphthacenes::Tetracyclines [Medical Subject Headings] Drug Resistance Multiple Bacterial polycyclic compounds Pharmacology (medical) Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::Carrier Proteins::Membrane Transport Proteins::ATP-Binding Cassette Transporters [Medical Subject Headings] Phylogeny Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Hydrolases::Amidohydrolases::beta-Lactamases [Medical Subject Headings] Minocycline Anti-Bacterial Agents Analytical Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Microbiological Techniques::Microbial Sensitivity Tests [Medical Subject Headings] Phenomena and Processes::Microbiological Phenomena::Bacterial Physiological Phenomena::Drug Resistance Bacterial::Drug Resistance Multiple Bacterial [Medical Subject Headings] Infectious Diseases Tetracyclines Gentamicin Efflux Chemicals and Drugs::Organic Chemicals::Amides::Lactams::beta-Lactams::Carbapenems [Medical Subject Headings] Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Therapeutic Uses::Anti-Infective Agents::Anti-Bacterial Agents [Medical Subject Headings] medicine.drug Acinetobacter Infections beta-Lactamasas Tetraciclinas CL 331002 Porins Microbial Sensitivity Tests Biology beta-Lactamases Microbiology Diseases::Bacterial Infections and Mycoses::Bacterial Infections::Gram-Negative Bacterial Infections::Moraxellaceae Infections::Acinetobacter Infections [Medical Subject Headings] Organisms::Bacteria::Gram-Negative Bacteria::Gram-Negative Aerobic Bacteria::Gram-Negative Aerobic Rods and Cocci::Moraxellaceae::Acinetobacter::Acinetobacter baumannii [Medical Subject Headings] Chemicals and Drugs::Heterocyclic Compounds::Heterocyclic Compounds 2-Ring::Quinolines::Quinolones [Medical Subject Headings] Antibiotic resistance Mechanisms of Resistance medicine Humans Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::Carrier Proteins::Membrane Transport Proteins::Ion Channels::Porins [Medical Subject Headings] Pharmacology Chemicals and Drugs::Carbohydrates::Glycosides::Aminoglycosides [Medical Subject Headings] Gene Expression Regulation Bacterial biochemical phenomena metabolism and nutrition biology.organism_classification bacterial infections and mycoses Chemicals and Drugs::Organic Chemicals::Amides::Lactams::beta-Lactams::Cephalosporins [Medical Subject Headings] Multiple drug resistance Aminoglycosides Carbapenems Quinolonas Phenomena and Processes::Genetic Phenomena::Phylogeny [Medical Subject Headings] bacteria ATP-Binding Cassette Transporters |
| Zdroj: | idUS. Depósito de Investigación de la Universidad de Sevilla instname |
| ISSN: | 1098-6596 |
| Popis: | We investigated the mechanisms of resistance to carbapenems, aminoglycosides, glycylcyclines, tetracyclines, and quinolones in 90 multiresistant clinical strains of Acinetobacter baumannii isolated from two genetically unrelated A. baumannii clones: clone PFGE-ROC-1 (53 strains producing the OXA-58 β-lactamase enzyme and 18 strains with the OXA-24 β-lactamase) and clone PFGE-HUI-1 (19 strains susceptible to carbapenems). We used real-time reverse transcriptase PCR to correlate antimicrobial resistance (MICs) with expression of genes encoding chromosomal β-lactamases (AmpC and OXA-51), porins (OmpA, CarO, Omp33, Dcap-like, OprB, Omp25, OprC, OprD, and OmpW), and proteins integral to six efflux systems (AdeABC, AdeIJK, AdeFGH, CraA, AbeM, and AmvA). Overexpression of the AdeABC system (level of expression relative to that by A. baumannii ATCC 17978, 30- to 45-fold) was significantly associated with resistance to tigecycline, minocycline, and gentamicin and other biological functions. However, hyperexpression of the AdeIJK efflux pump (level of expression relative to that by A. baumannii ATCC 17978, 8- to 10-fold) was significantly associated only with resistance to tigecycline and minocycline (to which the TetB efflux system also contributed). TetB and TetA(39) efflux pumps were detected in clinical strains and were associated with resistance to tetracyclines and doxycycline. The absence of the AdeABC system and the lack of expression of other mechanisms suggest that tigecycline-resistant strains of the PFGE-HUI-1 clone may be associated with a novel resistance-nodulation-cell efflux pump (decreased MICs in the presence of the inhibitor Phe-Arg β-naphthylamide dihydrochloride) and the TetA(39) system. |
| Databáze: | OpenAIRE |
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