Hyaluronic acid binding to CD44S is indiscriminate of molecular weight
| Autor: | Sun Jin Kim, Shawn C. Owen |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Surface Properties
Biophysics Breast Neoplasms Biochemistry Kilodalton Extracellular matrix 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cell surface receptor Cell Line Tumor Hyaluronic acid Humans Protein Isoforms Hyaluronic Acid Receptor 030304 developmental biology 0303 health sciences biology Molecular mass CD44 Cell Membrane Hyaluronic Acid Binding Cell Biology Extracellular Matrix Molecular Weight Hyaluronan Receptors chemistry 030220 oncology & carcinogenesis Antigens Surface biology.protein |
| Zdroj: | Biochimica et biophysica acta. Biomembranes. 1862(9) |
| ISSN: | 1879-2642 |
| Popis: | The ubiquitous presence of hyaluronic acid (HA) in the extracellular matrix (ECM) of both healthy and diseased tissues underscores its importance in human physiology. Previous studies suggest that HA can be used as a probe to qualitatively monitor cell surface levels of CD44 and other important HA receptors; however, these studies use mixtures of HA at various molecular weights. Using fluorescently labeled HA, we evaluated the apparent differences of low (25 kilodalton) and high (700 kilodalton) molecular weight HA interacting with breast cancer cell lines of varying levels of CD44. Our results confirm that CD44 expression and the apparent level of HA interaction correlates with molecular weight. Importantly, we show that HA only binds a small fraction of the major CD44 isoform, CD44S, on cell surfaces and that CD44S interactions account for |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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