High resolution allele genotyping and haplotype frequencies for NGS based HLA 11 loci of 5266 Hong Kong Chinese bone marrow donors
| Autor: | Wanling Yang, Zhongyi Liu, Wen Ma, Patrick Ip, Janette Kwok, Derek Middleton, Y.S. Chan, W.K. Chu, WH Tang, Cheuk-Kwong Lee |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Genotyping Techniques Immunology Population Human leukocyte antigen Biology 03 medical and health sciences 0302 clinical medicine Asian People Gene Frequency Bone Marrow HLA Antigens Immunology and Allergy Humans Registries Allele education Allele frequency Genotyping Alleles Genetic association Bone Marrow Transplantation Genetics education.field_of_study Histocompatibility Testing Haplotype Histocompatibility Antigens Class I Histocompatibility Antigens Class II High-Throughput Nucleotide Sequencing General Medicine Transplantation 030104 developmental biology Haplotypes Genetic Loci Hong Kong Unrelated Donors 030215 immunology |
| Zdroj: | Human immunology. 81(10-11) |
| ISSN: | 1879-1166 |
| Popis: | Next-generation sequencing (NGS) at the HLA-A, -B, -C, -DRB1, -DRB3/4/5, -DQA1, -DQB1, -DPA1, and -DPB1 loci was performed on 5,266 southern Chinese unrelated donors of the Hong Kong Bone Marrow Donor Registry. High-resolution HLA genotypes defined by full sequencing of class I loci and extended coverage of class II loci were attained to determine allele frequencies and estimate haplotype frequencies. This study provides allele and haplotype frequencies on 11 loci estimated for the first time in the Hong Kong Chinese population. These results describe extended haplotypes including the less frequently typed HLA-DPA1, -DPB1 and -DQA1 loci and distinctive haplotype associations. The present data are timely in that they allow the permissible matching in HLA-DPB1 for Chinese patients awaiting haematopoietic stem cell transplantation upon applying the latest requirement of NMDP matching guidelines. Overall, these results provide a useful reference source for population genetics studies, HLA-disease association studies and for improving donor recruitment and selection strategies of bone marrow registries. The allele and haplotype data are available in the Allele Frequencies Net Database under the population name ''Hong Kong Chinese HKBMDR, HLA 11 loci'' and the identifier (AFND3724) [1]. |
| Databáze: | OpenAIRE |
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