Vertical distribution of microbial communities in chromium-contaminated soil and isolation of Cr(Ⅵ)-Reducing strains
| Autor: | Yongji Huang, Hui Zhong, Yiran Yang, Bang Liu, Liang Hu, Guirong Su, Zhiguo He, Yang Yao |
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| Rok vydání: | 2019 |
| Předmět: |
Chromium
Health Toxicology and Mutagenesis 0211 other engineering and technologies Firmicutes chemistry.chemical_element 02 engineering and technology 010501 environmental sciences complex mixtures 01 natural sciences Actinobacteria Soil Metals Heavy RNA Ribosomal 16S Proteobacteria Soil Pollutants Ecosystem Phylogeny Soil Microbiology 0105 earth and related environmental sciences 021110 strategic defence & security studies Bacteria biology Microbiota Public Health Environmental and Occupational Health General Medicine biology.organism_classification Archaea Pollution Soil contamination Chloroflexi (class) chemistry Microbial population biology Environmental chemistry Environmental science Soil horizon |
| Zdroj: | Ecotoxicology and Environmental Safety. 180:242-251 |
| ISSN: | 0147-6513 |
| DOI: | 10.1016/j.ecoenv.2019.05.023 |
| Popis: | Soil ecosystems surrounding chromium slag undergo continuous harsh physicochemical conditions due to multiple heavy metals contamination. Previous studies of soil microbial communities mainly focused on surface soil layer, while little was known about the depth-related distributions of the microbial communities in chromium (Cr)-contaminated soil. In this study, a comprehensive analysis of depth-related distributions of microbial communities in Cr-contaminated soil was carried out by Illumina sequencing of 16s rRNA genes. The results revealed that bacterial diversities at 0 cm depth layer were significantly higher than those below 20 cm depths. And there was a remarkable difference in bacterial compositions along with the sampling depths especially for the dominant phyla of Proteobacteria, Actinobacteria, Chloroflexi and Fimicutes (p 0.05). While the archaea accounted for a relatively low proportion of the microbes and showed stability in the compositions with the predominant phyla of Thaumarchaeota and Euryarchaeota. The linear discriminate analysis (LDA) and effect size (LEfSe) analysis showed that there were thirty-seven kinds of biomarker microbes existing in the five soil layers with LDA threshold of 4.0, and each layer showed distinct microbial divisions, indicating that microbes with different biological functions might survive along with the sampling depths. The environmental variables including total chromium (Cr), Cr(Ⅵ), Mn, Ni, and Zn had considerable influences on microbial community composition in the contaminated soil. A total of 25 Cr(Ⅵ)-reducing strains were further isolated and identified, which were phylogenetically affiliated to Proteobacteria, Actinobacteria and Firmicutes. Among the isolated Cr(Ⅵ)-reducing strains, Bacillus stratosphericus was the first time to be reported with Cr(Ⅵ) reducing capacity. |
| Databáze: | OpenAIRE |
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