Isolation, identification, and cytotoxicity evaluation of phytochemicals from chloroform extract of Spathodea campanulata
| Autor: | Dipak Raut, Anita S. Wagh, Santosh R. Butle |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Cytotoxicity
lcsh:RS1-441 Umbelliferone 01 natural sciences lcsh:Pharmacy and materia medica 03 medical and health sciences chemistry.chemical_compound Column chromatography Viability assay 0303 health sciences Chloroform Leukemia biology Traditional medicine 030306 microbiology lcsh:RM1-950 biology.organism_classification In vitro 0104 chemical sciences 010404 medicinal & biomolecular chemistry African tulip tree lcsh:Therapeutics. Pharmacology chemistry Sulforhodamine assay Proton NMR Spathodea Chloroform extract |
| Zdroj: | Future Journal of Pharmaceutical Sciences, Vol 7, Iss 1, Pp 1-8 (2021) |
| ISSN: | 2314-7253 |
| Popis: | Background Spathodea campanulata P. Beauv. known as the African tulip tree has potential medicinal properties that have been shown traditionally for the treatment of various ailments. The aim of the present study was isolation, identification, and evaluation of the cytotoxic activity of phytochemicals from the chloroform extract of S. campanulata. Result Three compounds were isolated by using column chromatography and preparative TLC from chloroform extract of leaves of S. campanulata. The structures of the isolated compounds were elucidated by using spectroscopic methods, including, FTIR, ESI-TOF MS, 1H NMR, and 13C NMR spectroscopy. In vitro cytotoxic activity of compounds was evaluated by using SRB assay against human leukemia cancer cell lines (HL-60). Results were expressed in IC50 values. Stigmasta-5,22-dien-3-ol, octadecenamide, and umbelliferone were isolated and identified from chloroform extract. The isolated compounds showed cytotoxicity with decreasing cell viability in a dose-dependent manner, but it was found low as compared to positive control, i.e., Adriamycin against HL-60 cell lines. Conclusion The results indicate that isolated compounds, i.e., stigmasta-5,22-dien-3-ol (44.12μg/ml), octadecenamide (35.65μg/ml), and umbelliferone (80.60μg/ml) showed antiproliferative activity, but it was low compared to positive control Adriamycin (10.09 μg/ml). Also, according to our knowledge, this study is the first report on the isolation and identification of octadecenamide and umbelliferone from the leaves of S. campanulata. Graphical abstract |
| Databáze: | OpenAIRE |
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