Frequency of occurrence of HIV-1 dual infection in a Belgian MSM population
| Autor: | Chris Verhofstede, Leen Vancoillie, Delfien Staelens, Laura Hebberecht, Kenny Dauwe, Virginie Mortier, Marlies Schauvliege |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male RNA viruses Molecular biology DIVERSITY lcsh:Medicine RECOMBINATION HIV Infections HIV Envelope Protein gp120 medicine.disease_cause Pathology and Laboratory Medicine Men who have sex with men Database and Informatics Methods Sequencing techniques SEROCONVERTERS Belgium Immunodeficiency Viruses SEX WORKERS Prevalence Medicine and Health Sciences DNA sequencing lcsh:Science Phylogeny Data Management education.field_of_study Multidisciplinary High-Throughput Nucleotide Sequencing MEN Phylogenetic Analysis Genomics IMMUNODEFICIENCY-VIRUS TYPE-1 Middle Aged Phylogenetics Infectious Diseases Medical Microbiology Superinfection Viral Pathogens Cohort Viruses Pathogens SUBTYPE Sequence Analysis Transcriptome Analysis Research Article Adult Next-Generation Sequencing Computer and Information Sciences Bioinformatics Population Men WHO Have Sex with Men Biology Research and Analysis Methods Microbiology Deep sequencing Virus REGION 03 medical and health sciences SUPERINFECTION Retroviruses medicine Genetics Humans Pyrroles Evolutionary Systematics Homosexuality Male education Microbial Pathogens Retrospective Studies Taxonomy Evolutionary Biology lcsh:R DISEASE PROGRESSION Lentivirus Organisms Biology and Life Sciences HIV Computational Biology Retrospective cohort study Human Genetics Genome Analysis Virology Peptide Fragments 030104 developmental biology Molecular biology techniques People and Places HIV-1 lcsh:Q Population Groupings Sequence Alignment Sexuality Groupings |
| Zdroj: | PLoS ONE PLOS ONE PLoS ONE, Vol 13, Iss 4, p e0195679 (2018) |
| ISSN: | 1932-6203 |
| Popis: | Introduction: HIV-1 dual infection is a condition that results from infection with at least two HIV-1 variants from different sources. The scarceness of information on this condition is partly due to the fact that its detection is technically challenging. Using next-generation sequencing we defined the extent of HIV-1 dual infection in a cohort of men who have sex with men (MSM). Material & methods: Eighty-six MSM, diagnosed with HIV-1 subtype B infection between 2008 and 2013 were selected for next-generation sequencing of the HIV-1 envelope V3. Sequencing was performed on 2 plasma samples collected with an interval of > 6 months before the initiation of antiretroviral therapy. Maximum likelihood phylogenetic trees were inspected for dual infection, defined as the presence of two or more monophyletic clusters with >= 90% bootstrap support and a mean between-cluster genetic distance of >= 10%. To confirm dual infection, deep V3 sequencing of intermediate samples was performed as well as clonal sequencing of the HIV-1 protease-reverse transcriptase gene. Results: Five of the 74 patients (6.8%) for whom deep sequencing was successful, showed clear evidence of dual infection. In 4 of them, the second strain was absent in the first sample but occurred in subsequent samples. This was highly suggestive for superinfection. In 3 patients both virus variants were of subtype B, in 2 patients at least one of the variants was a subtype B/non-B recombinant virus. Conclusions: Dual infection was confirmed in 6.8% of MSM diagnosed with HIV-1 in Belgium. This prevalence is probably an underestimation, because stringent criteria were used to classify viral variants as originating from a new infection event. |
| Databáze: | OpenAIRE |
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