Discontinuous transcription of ribosomal DNA in human cells
| Autor: | Sami Kereïche, Pavel Studeny, Dušan Cmarko, Peter Trosan, Joao Victor Cabral, Eugene Smirnov, Kateřina Jirsová |
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| Rok vydání: | 2019 |
| Předmět: |
Transcription
Genetic Nucleolus Cell Cultured tumor cells Uridine Triphosphate Biochemistry Cell Signaling Transcription (biology) Transcriptional Control Middle Aged Cell biology Cold Temperature Nucleic acids medicine.anatomical_structure Ribosomal RNA Medicine Cell lines Biological cultures Cell Nucleolus Research Article Signal Transduction Cellular structures and organelles Science DNA transcription Biology Limbus Corneae Transfection DNA Ribosomal medicine RNA polymerase I Cadaver Genetics Humans Gene Regulation HeLa cells Non-coding RNA Ribosomal DNA Gene Uridine Aged Biology and life sciences Epithelial Cells DNA Cell cultures Research and analysis methods Kinetics RNA Ribosomal RNA Gene expression Ribosomes Software |
| Zdroj: | PLoS ONE PLoS ONE, Vol 15, Iss 3, p e0223030 (2020) |
| ISSN: | 1932-6203 |
| Popis: | Numerous studies show that various genes in all kinds of organisms are transcribed discontinuously, i.e. in short bursts or pulses with periods of inactivity between them. But it remains unclear whether ribosomal DNA (rDNA), represented by multiple copies in every cell, is also expressed in such manner. In this work, we synchronized the pol I activity in the populations of tumour derived as well as normal human cells by cold block and release. Our experiments with 5-fluorouridine (FU) and BrUTP confirmed that the nucleolar transcription can be efficiently and reversibly arrested at +4°C. Then using special software for analysis of the microscopic images, we measured the intensity of transcription signal (incorporated FU) in the nucleoli at different time points after the release. We found that the ribosomal genes in the human cells are transcribed discontinuously with periods ranging from 45 min to 75 min. Our data indicate that the dynamics of rDNA transcription follows the undulating pattern, in which the bursts are alternated by periods of rare transcription events. |
| Databáze: | OpenAIRE |
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