Cinnamon effectively inhibits the activity of leukemia stem cells
| Autor: | Guan X, Hu P, Ouyang Hm, Dong Xd, Su Mc, Yang Th, Pei Q, Zhao Rb, Li Zf, Zhang Cr, Lu J |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cinnamomum zeylanicum Myeloid Apoptosis Pharmacology Cell morphology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Genetics medicine Humans Molecular Biology Cell Proliferation Leukemia Plant Extracts Stem Cells Cell Cycle Cell Differentiation General Medicine Cell cycle medicine.disease 030104 developmental biology medicine.anatomical_structure chemistry 030220 oncology & carcinogenesis Caco-2 Cells Growth inhibition Stem cell K562 Cells K562 cells |
| Zdroj: | Genetics and Molecular Research. 15 |
| ISSN: | 1676-5680 |
| DOI: | 10.4238/gmr.15037662 |
| Popis: | Cinnamon is the main component of Sanyangxuedai, which is one of the effective traditional Chinese medicines for treating malignancies. Leukemia is a prevalent malignant disease that Sanyangxuedai has been used to treat. Although successful in several studies, there is a lack of solid evidence as to why Sanyangxuedai has an effect on leukemia, and little is known about the underlying mechanisms. In this study, the active ingredients of cinnamon were isolated, purified, and identified. The transwell transport pool formed with the Caco-2 cell model was used to filter the active ingredients of cinnamon by simulating the gastrointestinal barrier in vitro. Moreover, the cell morphology, cell cycle status, apoptosis status, and antigenic variation of the cell surface antigens were observed and measured in K562 cells after treatment with the active ingredients of cinnamon. Our results showed that 50-75 μM was a safe concentration of cinnamon extract for treatment of K562 cells for 72 h. The cinnamon extract caused growth inhibition of K562 cells. Cinnamon extract seemed to arrest the cells at the G1 stage and increased the apoptosis rate significantly. Interestingly, cinnamon extract treatment upregulated the expression of erythroid and myeloid differentiation antigens and downregulated that of the megakaryocytic differentiation antigens in a dose-dependent manner. Our findings indicate that cinnamon extract from Sanyangxuedai may be effective for treating leukemia. |
| Databáze: | OpenAIRE |
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