The effects of autologous bone marrow mesenchymal stem cell arterial perfusion on vascular repair and angiogenesis in osteonecrosis of the femoral head in dogs
| Autor: | Luwei Xiao, Nanze Yu, Bingjiang Xia, Peijian Tong, Bangjian He, Hongting Jin, Yan Shen |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A medicine.medical_specialty Angiogenesis Neovascularization Physiologic Mesenchymal Stem Cell Transplantation Transplantation Autologous Neovascularization Femoral head Dogs Femur Head Necrosis Medicine Animals Orthopedics and Sports Medicine Bone Marrow Transplantation Original Paper medicine.diagnostic_test business.industry Mesenchymal stem cell Angiography Femur Head Surgery Transplantation Vascular endothelial growth factor A Disease Models Animal medicine.anatomical_structure Treatment Outcome Orthopedic surgery Microvessels medicine.symptom business |
| Zdroj: | International orthopaedics. 36(12) |
| ISSN: | 1432-5195 |
| Popis: | The purpose of this study was to observe the effects of marrow mesenchymal stem cell (MSCs) arterial perfusion on vascular repair and angiogenesis in osteonecrosis of the femoral head (ONFH).Twelve healthy male adult Beagle dogs were randomly divided into two groups: group A (the control group) and group B (the MSCs arterial perfusion group). ONFH animal models were established by hip dislocation and liquid nitrogen. At the same time, MSCs were obtained, cultured and proliferated. After three weeks, arterial perfusion was performed in all animals. Group B was given 1 ml MSCs (5 × 10(6)-1 × 10(7)/ml), while 0.9 % normal saline was used in group A. After four weeks or eight weeks, the dogs were put to death. The changes of main arteries, the expression of vascular endothelial growth factor (VEGF), VEGF mRNA and microvessel density (MVD) of ONFH were observed. All the data were analysed by SPSS13.0.In digital subtraction angiography (DSA), after four or eight weeks of treatment, the quantity and diameter of the main arteries of the femoral head in group B were improved, compared to group A (P 0.05,P 0.01). Concerning histology and immunohistochemistry, after four or eight weeks of treatment, the expression of VEGF and MVD were significantly higher than that of group A (P 0.05, P 0.01). For real-time quantitative polymerase chain reaction (RT-PCR), after four or eight weeks of treatment, the expression of VEGF mRNA in group B was significantly higher than that of group A (P 0.05, P 0.01), and after eight weeks of treatment, the expression of VEGF mRNA were significantly higher than that of four-weeks treatment in group A (P0.01).MSCs arterial perfusion can promote vascular repair and angiogenesis and then improve blood supply and repair of femoral head. |
| Databáze: | OpenAIRE |
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