Inhibition of DNA Synthesis in Permeabilized Mouse P815 Mast Cells by Arabinofuranosyladenine 5′-Triphosphate
| Autor: | Ralf Schmidseder, Rudolf K. Zahn, Werner E.G. Müller, Werner Geurtsen, Armin Maidhof |
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| Rok vydání: | 1981 |
| Předmět: |
DNA Replication
Pharmacology Cell Membrane Permeability Leukemia Experimental Arabinonucleotides DNA synthesis Lysophosphatidylcholines chemical and pharmacologic phenomena DNA Neoplasm General Medicine Biology Molecular biology Cell biology Mice Eukaryotic Cells Infectious Diseases Oncology Drug Discovery Animals lipids (amino acids peptides and proteins) Pharmacology (medical) Mast Cells Vidarabine Phosphate Cells Cultured |
| Zdroj: | Chemotherapy. 27:61-67 |
| ISSN: | 1421-9794 0009-3157 |
| DOI: | 10.1159/000237956 |
| Popis: | P815 mast cells (mouse leukemic cells) have been successfully permeabilized to 9-beta-arabinofuranosyladenine 5'-triphosphate (araATP) using lysolecithin. The permeabilized cells remained viable according to the following criteria: (a) exclusion of trypan blue after replacing the lysolecithin with culture medium; (b) cell growth rate identical to control cultures, and (c) intact DNA-synthesizing complex in permeable cells. It was shown that permeabilized cells incorporate [3H]thymidine and [3H]deoxyadenosine-triphosphate (dATP) into DNA when the suspension is supplied with the appropriate substrates and cofactors. Permeable cells break down araATP to 9-beta-D-arabinofuranosyladenine 5'-diphosphate only slightly after incubation for 60 min; no further dephosphorylation to 9-beta-D-arabinofuranosyladenine 5'-monophosphate or 9-beta-D-arabinofuranosyladenine occurs. In kinetic experiments it was shown that the DNA-replicating complex of permeabilized cells is competitively inhibited by araATP with respect to dATP; Ki(araATP) was determined to be 0.6 microM and the Km(dATP), 0.9 microM. |
| Databáze: | OpenAIRE |
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