Derivation of human embryonic stem cell line Genea019
| Autor: | Divya Goel, Biljana Dumevska, Robert McKernan, Uli Schmidt, Teija T. Peura |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Homeobox protein NANOG Human Embryonic Stem Cells Karyotype Transplantation Heterologous Biology Andrology 03 medical and health sciences Mice medicine Animals Humans Blastocyst Induced pluripotent stem cell lcsh:QH301-705.5 Alleles Cells Cultured Medicine(all) Comparative Genomic Hybridization Teratoma Cell Differentiation General Medicine Cell Biology medicine.disease Cellular Reprogramming 030104 developmental biology medicine.anatomical_structure lcsh:Biology (General) Microscopy Fluorescence Cell culture embryonic structures Alkaline phosphatase Female Human embryonic stem cell line Transcription Factors Developmental Biology |
| Zdroj: | Stem Cell Research, Vol 16, Iss 2, Pp 397-400 (2016) |
| ISSN: | 1873-5061 |
| DOI: | 10.1016/j.scr.2016.02.008 |
| Popis: | The Genea019 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, through ICM outgrowth on inactivated feeders. The line showed pluripotent cell morphology and genomic analysis verified a 46, XX karyotype, female Allele pattern and unaffected Htt CAG repeat length, compared to HD affected sibling Genea020. Pluripotency of Genea019 was demonstrated with 75% of cells expressing Nanog, 89% Oct4, 48% Tra1-60 and 85% SSEA4, a Pluritest Pluripotency score of 22.97, Novelty score of 1.42, tri-lineage teratoma formation and Alkaline Phosphatase activity. The cell line was negative for Mycoplasma and any visible contamination. |
| Databáze: | OpenAIRE |
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