Osteoclast precursors do not express CD68: results from CD68 promoter-driven RANK transgenic mice

Autor: Marta Scatena, Melissa F. Jackson, Cecilia M. Giachelli
Rok vydání: 2016
Předmět:
0301 basic medicine
Myeloid
Cellular differentiation
Recombinant Fusion Proteins
Cathepsin K
Biophysics
Antigens
Differentiation
Myelomonocytic
Osteoclasts
Spleen
Bone Marrow Cells
Mice
Transgenic
Biochemistry
Tacrolimus
Article
Tacrolimus Binding Proteins
03 medical and health sciences
Mice
0302 clinical medicine
Structural Biology
Osteoclast
Antigens
CD
Genetics
medicine
Animals
Myeloid Cells
Promoter Regions
Genetic
Molecular Biology
Peritoneal Cavity
Mitogen-Activated Protein Kinase 1
Mitogen-Activated Protein Kinase 3
Receptor Activator of Nuclear Factor-kappa B
CD68
Activator (genetics)
Chemistry
Macrophages
Cell Differentiation
Cell Biology
Cell biology
030104 developmental biology
medicine.anatomical_structure
Gene Expression Regulation
Matrix Metalloproteinase 9
030220 oncology & carcinogenesis
Bone marrow
Signal transduction
Signal Transduction
Zdroj: FEBS letters. 591(5)
ISSN: 1873-3468
Popis: Macrophages and osteoclasts are thought to derive from CD68 lineage marker-positive common myeloid precursors. We used the CD68 promoter to drive an inducible receptor activator of NF-κB (iRANK) construct that selectively activates RANK signaling in myeloid cells in vivo. The cytoplasmic portion of RANK was fused to a mutant FK506 binding domain, which selectively binds the chemical inducer of dimerization AP20187 and initiates signaling. iRANK mRNA was expressed in macrophages isolated from peritoneal cavity, spleen-, and bone marrow-derived myeloid cells. Unexpectedly, AP20187 did not induce osteoclast formation in spleen- and bone marrow-derived myeloid cells. However, AP20187-dependent RANK signaling induced ERK1/2 phosphorylation and mRNA expression of MMP9 and CathepsinK in peritoneal macrophages. Importantly, CD68 was not expressed until day 3 and day 5 in bone marrow and spleen myeloid cells, respectively. Contrary to dogma, osteoclast precursors do not express the lineage marker CD68.
Databáze: OpenAIRE
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