The regiochemical distribution of positive charges along cholesterol polyamine carbamates plays significant roles in modulating DNA binding affinity and lipofection
| Autor: | Ian S. Blagbrough, Terry Baker, Michael Anthony William Eaton, Andrew J. Geall, Catherine Fiona Catterall |
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| Rok vydání: | 1999 |
| Předmět: |
Biophysics
CHO Cells DNA condensation Transfection Biochemistry chemistry.chemical_compound Molecular recognition Structural Biology Cholesterol polyamine carbamate Cricetinae Genetics Electrochemistry Polyamines Moiety Animals DNA binding affinity Ammonium Molecular Biology Chinese hamster ovary cell Cell Biology DNA DNA uptake efficiency Cationic lipid Cholesterol chemistry Cattle Lipopolyamine Lipoplex Polyamine Ethidium bromide |
| Zdroj: | FEBS letters. 459(3) |
| ISSN: | 0014-5793 |
| Popis: | We have quantified the effects of the regiochemical distribution of positive charges along the polyamine moiety in lipopolyamines for DNA molecular recognition. High affinity binding leads to charge neutralisation, DNA condensation and ultimately to lipofection. Binding affinities for calf thymus DNA were determined using an ethidium bromide displacement assay and condensation was detected by changes in turbidity using light scattering. The in vitro transfection competence of cholesterol polyamine carbamates was measured in CHO cells. In the design of DNA condensing and transfecting agents for non-viral gene therapy, the interrelationship of ammonium ions, not just their number, must be considered. |
| Databáze: | OpenAIRE |
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