Circadian regulation of cell cycle and apoptosis proteins in mouse bone marrow and tumor
| Autor: | Elisabeth Filipski, Rune Smaaland, Paolo Sassone-Corsi, Teresa G. Granda, Nicolas Cermakian, Francis Lévi, Xu-Hui Liu |
|---|---|
| Přispěvatelé: | Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Louis Pasteur - Strasbourg I |
| Rok vydání: | 2004 |
| Předmět: |
MESH: Taxoids
Circadian clock CLOCK Proteins Apoptosis Cell Cycle Proteins MESH: Cell Cycle Docetaxel Biochemistry Mice MESH: Mammary Neoplasms Animal 0302 clinical medicine Bone Marrow MESH: Basic Helix-Loop-Helix Transcription Factors Basic Helix-Loop-Helix Transcription Factors MESH: Animals bcl-2-Associated X Protein Mice Inbred C3H 0303 health sciences Cell Cycle ARNTL Transcription Factors Nuclear Proteins Period Circadian Proteins MESH: Transcription Factors Cell cycle MESH: Gene Expression Regulation Circadian Rhythm CLOCK medicine.anatomical_structure Proto-Oncogene Proteins c-bcl-2 Mice Inbred DBA 030220 oncology & carcinogenesis MESH: Cell Division Taxoids MESH: Bone Marrow Cell Division Biotechnology G2 Phase medicine.medical_specialty MESH: Cell Line Tumor MESH: Trans-Activators Mammary Neoplasms Animal Adenocarcinoma Biology 03 medical and health sciences MESH: Cell Cycle Proteins Bcl-2-associated X protein MESH: Mice Inbred C57BL Cell Line Tumor Internal medicine Genetics medicine Animals MESH: bcl-2-Associated X Protein MESH: Circadian Rhythm Circadian rhythm MESH: Mice Inbred C3H MESH: Mice Molecular Biology 030304 developmental biology MESH: Mice Inbred DBA MESH: Apoptosis MESH: Adenocarcinoma [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Mice Inbred C57BL MESH: G2 Phase Endocrinology Gene Expression Regulation MESH: Proto-Oncogene Proteins c-bcl-2 Trans-Activators biology.protein Bone marrow MESH: Nuclear Proteins Transcription Factors |
| Zdroj: | FASEB Journal FASEB Journal, Federation of American Society of Experimental Biology, 2005, 19 (2), pp.304-6. ⟨10.1096/fj.04-2665fje⟩ |
| ISSN: | 1530-6860 0892-6638 |
| DOI: | 10.1096/fj.04-2665fje |
| Popis: | International audience; Proapoptotic drugs such as docetaxel displayed least toxicity and highest antitumor efficacy following dosing during the circadian rest phase in mice, suggesting that cell cycle and apoptotic processes could be regulated by the circadian clock. In study 1, mouse bone marrow and/or tumor were obtained every 4 h for 24 h in C3H/HeN mice with or without MA13/C mammary adenocarcinoma in order to determine the circadian patterns in cell-cycle phase distribution and BCL-2 anti-apoptotic protein expression. In study 2, mouse bone marrow from B6D2F1 mice was sampled every 3 h for 24 h in order to confirm the BCL-2 rhythm and to study its relation with 24 h changes in the expression of proapoptotic BCL-2-associated X protein (BAX) protein and clock genes mPer2, mBmal1, mClock, and mTim mRNAs. The rhythms in G1-, S- or G2/M-phase cells were shifted in tumor compared with bone marrow. In the tumor, the mean proportion of G2/M-phase cells increased by 75% from late rest to late activity span (P from cosinor = 0.001). No 24 h rhythm was found for BCL-2 in tumors. In contrast to this, in the bone marrow, mean BCL-2 expression varied 2.8-fold in B6D2F1 mice (P=0.025) and 3- or 4.5-fold in tumor-bearing and nontumor-bearing C3H/HeN mice, with a peak during the early rest span (P=0.024 and P |
| Databáze: | OpenAIRE |
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