Emerging highly pathogenic H5 avian influenza viruses in France during winter 2015/16: phylogenetic analyses and markers for zoonotic potential
| Autor: | Aurélie Le Prioux, Evelyne Lemaitre, Edouard Hirchaud, Chantal Allée, Sylvie van der Werf, Martine Cherbonnel-Pansart, Fabrice Touzain, Hélène Quenault, Nicolas Eterradossi, Carole Guillemoto, Véronique Jestin, Alexandre Fediaevsky, Marie-Odile Le Bras, Hélène Gares, Pascale Massin, Yannick Blanchard, Eric Niqueux, Céline Courtillon, Katell Ogor, François-Xavier Briand, Audrey Schmitz, Patrick Daniel, Cécile Guillou-Cloarec |
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| Přispěvatelé: | Laboratoire de Ploufragan-Plouzané-Niort [ANSES], Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Université Bretagne Loire (UBL), Laboratoire départemental d'analyse et de recherche de Dordogne (LDAR 24), Conseil départemental de la Dordogne - Service de l'archéologie, Laboratoires des Pyrénées et des Landes, Direction Générale de l'Alimentation (DGAL), Ministère de l'agriculture, de l'agroalimentaire et de la forêt, Génétique Moléculaire des Virus à ARN - Molecular Genetics of RNA Viruses (GMV-ARN (UMR_3569 / U-Pasteur_2)), Institut Pasteur [Paris] (IP)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Genes Viral Epidemiology animal diseases [SDV]Life Sciences [q-bio] highly pathogenic Hemagglutinin Glycoproteins Influenza Virus Avian influenza medicine.disease_cause phylogeny Polymerase Chain Reaction Genome Poultry Disease Outbreaks law.invention 0403 veterinary science law Pandemic Polymerase chain reaction Genetics Phylogenetic tree virus diseases 04 agricultural and veterinary sciences Ducks H5 Zoonotic Potential RNA Viral France Influenza A Virus H5N2 Subtype Research Article 040301 veterinary sciences Molecular Sequence Data Virulence Biology Virus Birds 03 medical and health sciences Phylogenetics Virology medicine Animals Humans Poultry Diseases Influenza A Virus H5N1 Subtype Public Health Environmental and Occupational Health Sequence Analysis DNA Influenza A virus subtype H5N1 030104 developmental biology Influenza in Birds Chickens |
| Zdroj: | Eurosurveillance Eurosurveillance, 2017, 22 (9), ⟨10.2807/1560-7917.ES.2017.22.9.30473⟩ |
| ISSN: | 1560-7917 1025-496X |
| DOI: | 10.2807/1560-7917.ES.2017.22.9.30473⟩ |
| Popis: | International audience; Several new highly pathogenic (HP) H5 avian influenza virus (AIV) have been detected in poultry farms from south-western France since November 2015, among which an HP H5N1. The zoonotic potential and origin of these AIVs immediately became matters of concern. One virus of each subtype H5N1 (150169a), H5N2 (150233) and H5N9 (150236) was characterised. All proved highly pathogenic for poultry as demonstrated molecularly by the presence of a polybasic cleavage site in their HA protein – with a sequence (HQRRKR/GLF) previously unknown among avian H5 HPAI viruses – or experimentally by the in vivo demonstration of an intravenous pathogenicity index of 2.9 for the H5N1 HP isolate. Phylogenetic analyses based on the full genomes obtained by NGS confirmed that the eight viral segments of the three isolates were all part of avian Eurasian phylogenetic lineage but differed from the Gs/Gd/1/96-like lineage. The study of the genetic characteristics at specific amino acid positions relevant for modulating the adaptation to and the virulence for mammals showed that presently, these viruses possess most molecular features characteristic of AIV and lack some major characteristics required for efficient respiratory transmission to or between humans. The three isolates are therefore predicted to have no significant pandemic potential. |
| Databáze: | OpenAIRE |
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